The examination of the complex cell biology of the human malaria parasite Plasmodium falciparum usually relies on the time-consuming generation of transgenic parasites. Here, metabolic labeling and click chemistry are employed as a fast transfection-independent method for the microscopic examination of protein S-palmitoylation, an important post-translational modification during the asexual intraerythrocytic replication of P. falciparum. Applying various microscopy approaches such as confocal, single-molecule switching, and electron microscopy, differences in the extent of labeling within the different asexual developmental stages of P. falciparum and the host erythrocytes over time are observed.

中文翻译：

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恶性疟原虫感染的红细胞中的棕榈酰化蛋白：点击化学和代谢标记的研究。

对人类疟原虫恶性疟原虫的复杂细胞生物学的检查通常依赖于耗时的转基因寄生虫的产生。在这里，代谢标记和点击化学被用作一种快速转染独立方法，用于蛋白质 S-棕榈酰化的显微镜检查，这是恶性疟原虫无性红细胞内复制过程中的一种重要的翻译后修饰。应用各种显微镜方法，如共聚焦、单分子切换和电子显微镜，观察到随着时间的推移，恶性疟原虫和宿主红细胞的不同无性发育阶段内标记程度的差异。