DEAD-box RNA helicases regulate growth, development, and stress response of plants. In this study, a gene encoding a DEAD-box RNA helicase, GhRH32, was identified in upland cotton (Gossypium hirsutum L.). Gene silencing experiments demonstrated that GhRH32 functions as a negative regulator in cold tolerance. ATPase activity and ATP content analysis revealed that GhRH32 regulates ATP supply. Subcellular localization analysis showed that GhRH32 is targeted to the nucleus. Furthermore, we found that GhRH32 interacts with GhCBF, and the expression of CBF pathway genes increased in plants that were subjected to virus induced gene silencing (VIGS). Taken together, our results indicate that GhRH32 could be a potential molecular target to improve cold tolerance in plants.

中文翻译：

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GhRH32 负向调节陆地棉 (Gossypium hirsutum L.) 的耐寒性

DEAD-box RNA 解旋酶调节植物的生长、发育和应激反应。在这项研究中，在陆地棉 (Gossypium hirsutum L.) 中鉴定了一个编码 DEAD-box RNA 解旋酶 GhRH32 的基因。基因沉默实验表明 GhRH32 在耐寒性中起负调节作用。ATPase 活性和 ATP 含量分析表明 GhRH32 调节 ATP 供应。亚细胞定位分析显示 GhRH32 靶向细胞核。此外，我们发现 GhRH32 与 GhCBF 相互作用，并且在经历病毒诱导基因沉默 (VIGS) 的植物中，CBF 通路基因的表达增加。总之，我们的结果表明 GhRH32 可能是提高植物耐寒性的潜在分子靶标。