We introduced a new molecular imprinted polymers (MIPs) monolithic column of Mycophenolate mofetil (MMF) for HPLC. The monolithic column was prepared using MMF as the template molecule, methcrylic acid (MAA) as the functional monomer, vinyl ester resin as the crosslinking agent and AIBN as the initiator. HPLC was used to study the binding and separation performance of MIPs for template MMF and its cleavage product Mycophenolic acid (MPA). For chromatographic analysis the effects on the separation, mobile phase composition, flowrate and pH value were discussed respectively. Under the selected chromatographic conditions, MMF and MPA could achieve base line separation within 4 min with a separation degree of 2.68. Moreover, the linearity of the calibration plot was evaluated of seven spiked samples (2.0–100.0 µg·mL −1 ) in plasma, and the limit of detection (LOD) for MMF in plasma and the stability of the monolithic column were also studied. Twelve-hour pharmacokinetic profile of both MMF and MPA for a renal transplant recipient receiving oral dosing of 1000 mg MMF was investigated. Results indicated that this method could be applied for separation and screening of MMF and MPA, It provided a simple and fast method for assaying this drug in plasma sample.

中文翻译：

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新型（乙烯基酯）树脂分子印迹整体柱快速分离和筛选霉酚酸酯和霉酚酸

我们介绍了一种用于 HPLC 的新型霉酚酸酯 (MMF) 分子印迹聚合物 (MIP) 整体柱。以MMF为模板分子，甲基丙烯酸（MAA）为功能单体，乙烯基酯树脂为交联剂，AIBN为引发剂，制备整体柱。HPLC 用于研究 MIPs 对模板 MMF 及其裂解产物霉酚酸 (MPA) 的结合和分离性能。对于色谱分析，分别讨论了对分离、流动相组成、流速和pH值的影响。在选定的色谱条件下，MMF 和 MPA 可在 4 min 内实现基线分离，分离度为 2.68。此外，对血浆中的七个加标样品 (2.0–100.0 µg·mL -1 ) 评估了校准曲线的线性，还研究了血浆中 MMF 的检测限 (LOD) 和整体柱的稳定性。研究了接受口服 1000 mg MMF 的肾移植受者的 MMF 和 MPA 的 12 小时药代动力学特征。结果表明，该方法可用于MMF和MPA的分离和筛选，为血浆样品中该药物的测定提供了一种简便、快速的方法。