A multi-step aqueous and non-aqueous extraction procedure was applied to recovery bio-molecules from Phaeodactylum tricornutum. The process include that physical pre-treatments (high voltage electrical discharges (HVED, 40 kV/cm, 1–8 ms, HVED samples) or high pressure homogenization (HPH, 1200 bar, 10 passes, P samples)) (1st step); aqueous extraction (2nd step); pigments extraction in ethanol (3rd step); and lipids extraction in CHCl₃/MeOH (4th step). The extractability of ionics, carbohydrates, proteins, pigments and lipids for untreated, HVED and P samples were evaluated. The results evidenced that HVED allowed selective extraction of water soluble ionic products at 1st and 2nd steps. The maximum ionic concentrations were found for HVED samples. However, P samples resulted in higher contents of extracted components as compared to HVED samples (≈1.5-fold of carbohydrates, ≈2.5-fold of proteins, ≈5-fold of carotenoids, and ≈3-fold of chlorophylls). Moreover, the non-aqueous extraction (3rd and 4th steps) allowed supplementary extraction of pigments and lipids.

Industrial relevance

Pre-treatments by high voltage electrical discharge (HVED) or high pressure homogenization (HPH) allowed effective extraction of different bio-molecules from microalga biomass. The combination of HVED/HPH, aqueous and non-aqueous extraction allowed selective recovery of target molecules. The paper also presents a multistage extraction procedure allowed reducing the amount of toxic solvents, increasing the extraction efficiency, and reducing the energy consumption. These approaches opened the doors to high-efficiency recovery of valuable compounds from microalgae relevant for industrial application.

中文翻译：

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多级水和非水提取微藻藻中的生物分子

将多步水和非水提取程序应用于从三角果蝇（Phaeodyylum tricornutum）中回收生物分子。该过程包括物理预处理（高压放电（HVED，40 kV / cm，1-8 ms，HVED样品）或高压均质化（HPH，1200 bar，10次通过，P样品））（第一步） ; 水提取（第二步）；在乙醇中提取颜料（第三步）；和HCl中的脂质和脂质提取₃/ MeOH（第4步）。评估了未经处理的HVED和P样品中离子，碳水化合物，蛋白质，颜料和脂质的可萃取性。结果证明，HVED允许在第一步和第二步中选择性提取水溶性离子产品。发现了HVED样品的最大离子浓度。然而，与HVED样品相比，P样品导致提取的成分含量更高（碳水化合物约为1.5倍，蛋白质约为2.5倍，类胡萝卜素约为5倍，叶绿素约为3倍）。此外，非水提取（第3和第4步）可以补充提取色素和脂质。

行业相关性

通过高压放电（HVED）或高压均质化（HPH）进行的预处理可以有效地从微藻生物质中提取不同的生物分子。HVED / HPH，水和非水萃取相结合，可以选择性回收目标分子。本文还提出了一种多级萃取程序，可以减少有毒溶剂的量，提高萃取效率并降低能耗。这些方法为从与工业应用相关的微藻中高效回收有价值的化合物打开了大门。