The regulation of porcine subcutaneous (SC) and intramuscular (IM) fat deposition significantly affects pork quality and the lean meat percentage of the carcass, respectively. The adipokine C1q/tumor necrosis factor-related protein 6 (CTRP6), plays a significant role in regulating animal fat deposition. The purpose of this study was to understand the effects of CTRP6 gene knockdown in IM and SC adipocytes by RNA-seq analysis. A total of 1830 and 2936 differentially expressed genes (DEGs) were identified in SC and IM adipocytes, respectively. 844 were down- and 2092 were upregulated in SC adipocytes, while 648 were down- and 1182 were upregulated in IM adipocytes. Furthermore, 1778 DEGs were detected only in SC adipocytes, 672 DEGs only in IM adipocytes, and 1158 DEGs in both types of adipocytes. GO analysis indicated that DEGs involved in adipocyte differentiation were significantly enriched in both SC and IM adipocytes following treatment with CTRP6-siRNA. Moreover, KEGG pathway enrichment analysis revealed differences of metabolic regulation between IM and SC adipocytes. With CTRP6-silencing, the signaling pathways related to Ras and arachidonic acid metabolism were significantly enriched in IM adipocytes, while four other signaling pathways, encompassing the TNF, MAPK, p53 and adipokine pathway were specifically enriched in SC adipocytes. Interestingly, the effect of CTRP6-siRNA treatment was attenuated by the specific Ras activator ML-097 in IM adipocytes, while the specific p53 activator SJ-172550 had the corresponding effect in SC adipocytes. Altogether, we suggest that CTRP6 may be a differential regulator of the development and metabolism of IM and SC adipose tissues.

中文翻译：

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敲低CTRP6可以通过不同的信号传导途径减少猪肌肉和皮下脂肪的沉积。

猪皮下（SC）和肌内（IM）脂肪沉积的调节分别显着影响猪肉质量和car体瘦肉率。脂肪因子C1q /肿瘤坏死因子相关蛋白6（CTRP6）在调节动物脂肪沉积中起重要作用。这项研究的目的是通过RNA序列分析了解CTRP6基因敲低对IM和SC脂肪细胞的影响。在SC和IM脂肪细胞中分别鉴定出总共1830和2936个差异表达基因（DEG）。SC脂肪细胞中有844个下调，2092个上调，而IM脂肪细胞中有648个下调，1182个上调。此外，仅在SC脂肪细胞中检测到1778DEG，仅在IM脂肪细胞中检测到672DEG，在两种类型的脂肪细胞中检测到1158DEG。GO分析表明，用CTRP6-siRNA处理后，参与脂肪细胞分化的DEG在SC和IM脂肪细胞中均显着富集。此外，KEGG通路富集分析揭示了IM和SC脂肪细胞之间的代谢调控差异。通过CTRP6沉默，IM脂肪细胞中显着丰富了与Ras和花生四烯酸代谢相关的信号通路，而SC脂肪细胞中特别丰富了包括TNF，MAPK，p53和脂肪因子在内的其他四个信号通路。有趣的是，IM脂肪细胞中特定的Ras激活剂ML-097减弱了CTRP6-siRNA处理的效果，而SC脂肪细胞中特定的p53激活剂SJ-172550具有相应的作用。共，