In this work, we analyzed the diversity of seventy-six bacteria isolated from Pea and faba bean nodules in two regions of Morocco. The molecular diversity was realized using the analysis of the sequences of 16S rRNA and six housekeeping genes (recA, glnII, atpD, dnaK, rpoB and gyrB) and two symbiotic genes (nodA and nodC).

The phylogeny of the 16S rRNA gene sequences revealed that all strains belong to the genus Rhizobium, being related to the type strains of R. leguminosarum, R. laguerreae, R. indigoferae, R. anhuiense and R. acidisoli. The housekeeping genes phylogenies showed that some strains formed a subclade distinct from the rhizobial species that usually nodulate Vicia faba and Pisum sativum which are closely related to R. acidisoli FH23 with sequence similarity of 98.3%.

Analysis of the PGPR activities of the different isolates showed that the strains related to R. laguerreae were able to solubilize phosphates and to produce siderophores and auxin phytohormone. However, R. acidisoli strain F40D2 was unable to solubilize phosphates although they produce siderophores and IAA.

The phylogenetic analysis of the nodA and nodC sequences showed that all isolated strains were closely related with the strains of symbiovar viciae. The nodulation tests confirmed the ability to nodulate V. faba and P. sativum but not Cicer arietinum or Phaseolus vulgaris. Hence, in Morocco P. sativum is nodulated by R. laguerreae; whereas V. faba is nodulated by R. laguerreae and the symbiovar viciae of R. acidisoli which has been not previously described in this species.

在这项工作中，我们分析了从摩洛哥两个地区的豌豆和蚕豆根瘤中分离出来的76种细菌的多样性。通过分析16S rRNA和六个管家基因（recA，glnII，atpD，dnaK，rpoB和gyrB）和两个共生基因（nodA和nodC）来实现分子多样性。

16S rRNA基因序列的系统发育揭示所有菌株属于属根瘤菌，被有关的型菌株豌豆根瘤菌，R. laguerreae，R. indigoferae，R. anhuiense和R. acidisoli。管家基因系统发育研究表明，一些菌株形成了不同于根瘤菌的亚群，后者通常结瘤于蚕豆和豌豆，与酸果蝇FH23密切相关，序列相似性为98.3％。

对不同菌株的PGPR活性的分析表明，与R. laguerreae有关的菌株能够溶解磷酸盐并产生铁载体和生长素植物激素。然而，酸伊索菌菌株F40D2尽管能产生铁载体和IAA，却无法溶解磷酸盐。

对nodA和nodC序列的系统发育分析表明，所有分离的菌株均与共生蚕豆菌株密切相关。该结瘤试验证实的能力，形成根瘤蚕豆和P.豌豆但不鹰嘴豆或菜豆。因此，在摩洛哥P.豌豆由根瘤R. laguerreae ; 而蚕豆是由拉格雷尔氏菌和酸异丝状菌的共生菌丝虫形成的，这在该物种中以前没有描述过。