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How consistent is RAD-seq divergence with DNA-barcode based clustering in insects?
Molecular Ecology Resources ( IF 7.7 ) Pub Date : 2020-04-27 , DOI: 10.1111/1755-0998.13178
Marie Cariou 1 , Hélène Henri 1 , Sonia Martinez 1 , Laurent Duret 1 , Sylvain Charlat 1
Affiliation  

Promoted by the barcoding approach, mitochondrial DNA is more than ever used as a molecular marker to identify species boundaries. Yet, it has been repeatedly argued that it may be poorly suited for this purpose, especially in insects where mitochondria are often associated with invasive intracellular bacteria that may promote their introgression. Here, we inform this debate by assessing how divergent nuclear genomes can be when mitochondrial barcodes indicate very high proximity. To this end, we obtained RAD‐seq data from 92 barcode‐based species‐like units (operational taxonomic units [OTUs]) spanning four insect orders. In 100% of the cases, the observed median nuclear divergence was lower than 2%, a value that was recently estimated as one below which nuclear gene flow is not uncommon. These results suggest that although mitochondria may occasionally leak between species, this process is rare enough in insects to make DNA barcoding a reliable tool for clustering specimens into species‐like units.

中文翻译:

RAD-seq 分歧与基于 DNA 条形码的昆虫聚类的一致性如何?

在条形码方法的推动下,线粒体 DNA 比以往任何时候都更多地用作分子标记来识别物种边界。然而,一再争论它可能不适合此目的,特别是在昆虫中,线粒体通常与可能促进其基因渗入的侵入性细胞内细菌有关。在这里,我们通过评估当线粒体条形码指示非常接近时核基因组的差异程度来为这场辩论提供信息。为此,我们从跨越四个昆虫目的 92 个基于条形码的物种类单位(操作分类单位 [OTU])中获得了 RAD-seq 数据。在 100% 的情况下,观察到的中值核发散低于 2%,最近估计该值低于该值,低于该值核基因流并不少见。
更新日期:2020-04-27
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