Gastrointestinal stromal tumor (GIST) is a common mesenchymal tumor in the gastrointestinal tract. Prostate cancer associated transcript 6 (PCAT6) is a long noncoding RNA (lncRNA) and plays a pivotal role in tumor formation. Present study was designed to explore the function of PCAT6 in GIST. Ki67 staining, colony formation and trypan blue staining assays revealed that PCAT6 boosted GIST cell proliferation but inhibited cell apoptosis. Also, sphere formation assay and Western blot uncovered the promoting role of PCAT6 in GIST stemness. Then, we identified that PCAT6 could activate Wnt/β-catenin pathway. And the tumor facilitator role of Wnt/β-catenin pathway was validated in the rescue assays. Next, miR-143-3p was identified as the downstream microRNA of PCAT6. Moreover, miR-143-3p itself served as a tumor suppressor in GIST. Subsequently, peroxiredoxin 5 (PRDX5) was verified as the target of miR-143-3p. PCAT6 promoted GIST cell proliferation and stemness via sponging miR-143-3p to upregulate PRDX5. In a word, PCAT6 promoted GIST cell proliferation and stemness but inhibited cell apoptosis via competing endogenous RNA pattern and activation of Wnt pathway, which might contribute to GIST treatment.

中文翻译：

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PCAT6通过上调PRDX5和激活Wnt通路介导胃肠道间质瘤的细胞生物学功能。

胃肠道间质瘤（GIST）是胃肠道中常见的间质肿瘤。前列腺癌相关转录本6（PCAT6）是一个长的非编码RNA（lncRNA），在肿瘤形成中起关键作用。本研究旨在探讨PCAT6在GIST中的功能。Ki67染色，集落形成和锥虫蓝染色分析表明，PCAT6促进GIST细胞增殖，但抑制细胞凋亡。此外，球形成分析和蛋白质印迹揭示了PCAT6在GIST茎中的促进作用。然后，我们确定PCAT6可以激活Wnt /β-catenin途径。Wnt /β-catenin途径的肿瘤促进作用在抢救试验中得到了验证。接下来，miR-143-3p被鉴定为PCAT6的下游microRNA。此外，miR-143-3p本身在GIST中起着抑癌作用。后来，已验证过氧化物酶5（PRDX5）是miR-143-3p的靶标。PCAT6通过使miR-143-3p海绵化以上调PRDX5来促进GIST细胞增殖和干性。简而言之，PCAT6通过竞争性内源RNA模式和Wnt途径的激活来促进GIST细胞增殖和干性，但抑制细胞凋亡，这可能有助于GIST治疗。