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The unfolded protein response modulates the autophagy-mediated egg production in the mosquito Aedes aegypti.
Insect Molecular Biology ( IF 2.6 ) Pub Date : 2020-05-04 , DOI: 10.1111/imb.12645
S-C Weng,S-H Shiao

Mosquitoes must feed on vertebrate blood for egg development. As a consequence, some mosquito species are vectors for pathogens that cause devastating diseases in humans. Hence, understanding the mechanisms that control egg developmental cycles is important for developing novel approaches for the control of mosquito‐borne diseases. The unfolded protein response (UPR) is a cellular stress response related to endoplasmic reticulum (ER) stress. The UPR is activated in response to an accumulation of unfolded or misfolded proteins in the ER. Massive proteins have been shown to be produced during egg development, and it is obvious that unfolded or misfolded proteins may arise during vitellogenesis. It has been shown that autophagy in the mosquito fat body plays a central role in the progression of gonadotrophic cycles in the mosquito Aedes aegypti. However, the molecular mechanisms underlying the induction of UPR and the correlation between UPR and autophagy remain unclear. Here, we demonstrate that autophagy is activated during vitellogenesis and that the activation of autophagy is correlated with the UPR. We also show that the expressions of UPR and autophagy can be induced in an in vitro fat body culture system through an amino acid treatment. In addition, the expressions of UPR, autophagy‐specific markers and vitellogenin were also induced during dithiothreitol treatment. Interestingly, the silencing of UPR‐related genes significantly reduced the expression of autophagy‐specific markers and inhibited mosquito fecundity. Taken together, we conclude that autophagy‐mediated egg production in the mosquito A. aegypti is regulated by UPR.

中文翻译:

展开的蛋白质反应调节了埃及伊蚊的自噬介导的卵的产生。

蚊子必须以脊椎动物的血液为食,以发育卵子。结果,一些蚊子是引起人类毁灭性疾病的病原体的载体。因此,了解控制卵子发育周期的机制对于开发控制蚊媒疾病的新方法很重要。展开的蛋白质反应(UPR)是与内质网(ER)应激相关的细胞应激反应。UPR响应于ER中未折叠或错误折叠的蛋白质的积累而被激活。大量蛋白质被证明在卵发育过程中产生,很明显在卵黄形成过程中可能会出现未折叠或错误折叠的蛋白质。研究表明,蚊子脂肪体内的自噬在蚊子的性腺营养循环中起着核心作用。埃及伊蚊。然而,UPR诱导的分子机制以及UPR与自噬之间的相关性仍不清楚。在这里,我们证明自噬在卵黄发生过程中被激活,并且自噬的激活与UPR相关。我们还表明,通过氨基酸处理可以在体外脂肪培养系统中诱导UPR和自噬的表达。此外,在二硫苏糖醇治疗期间还诱导了UPR,自噬特异性标志物和卵黄蛋白原的表达。有趣的是,与UPR相关的基因的沉默显着降低了自噬特异性标志物的表达并抑制了蚊子的繁殖力。综上所述,我们得出的结论是,自噬介导的蚊子产卵埃及伊蚊由UPR监管。
更新日期:2020-05-04
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