Abiotic factors like salinity, drought and cold affect agricultural productivity substantially worldwide. NAC (NAM, ATAF1/2 and CUC2) family transcription factors have been shown to play important roles in biotic and abiotic stress responses in many plant species. Our previous studies have shown that AmNAC1, a gene from mangrove Avicennia marina, is differentially regulated in response to salt, drought and cold stresses and salicylic acid treatment. To gain more insights into the function of AmNAC1 in abiotic stress, AmNAC1 was heterologously expressed in yeast and tobacco. Heterologous expression of AmNAC1 in yeast (Saccharomyces cerevisiae) resulted in improved tolerance to salinity (NaCl and LiCl), alkalinity (NaHCO₃) stresses. In addition, transgenic yeast exhibited enhanced tolerance compared to control when subjected to high (50 °C) and freezing (− 20 °C) temperature. Similarly, overexpression of AmNAC1 in tobacco using the constitutive promoter (2xCaMV) demonstrated increased tolerance to abiotic stresses such as salinity, drought and cold. Specifically, compared to wild type (WT) and vector control (VC), AmNAC1 transgenic lines exhibited higher germination, increased root length and better survival rates at the whole plant level. Collectively, these results indicate a role for AmNAC1 in multiple abiotic stress tolerance.

诸如盐度，干旱和寒冷等非生物因素在很大程度上影响着全世界的农业生产力。NAC（NAM，ATAF1 / 2和CUC2）家族转录因子已显示在许多植物物种的生物和非生物胁迫响应中起重要作用。我们以前的研究表明，来自红树林Avicennia marina的基因AmNAC1在盐，干旱和寒冷胁迫以及水杨酸处理中受到不同的调节。为了获得更深入地了解各功能AmNAC1非生物胁迫，AmNAC1被异源酵母和烟草表达。AmNAC1在酵母（Saccharomyces cerevisiae）中的异源表达）提高了盐度（NaCl和LiCl），碱度（NaHCO ₃）胁迫的耐受性。此外，转基因酵母在高温（50°C）和冷冻（-20°C）下与对照相比显示出更高的耐受性。同样，使用组成型启动子（2xCaMV）在烟草中过量表达AmNAC1表现出对非生物胁迫（如盐度，干旱和寒冷）的耐受性增加。具体而言，与野生型（WT）和载体对照（VC）相比，AmNAC1转基因品系在整个植物水平上表现出更高的发芽率，增加的根长和更好的存活率。这些结果共同表明AmNAC1在多种非生物胁迫耐受性中的作用。