The insulin-like androgenic gland (IAG) gene is well known in male crustacean, and it is a key regulator in male sexual differentiation and maintaining the male sexual characteristic. The neo-female can be produced by silencing the MrIAG (Macrobrachium rosenbergii Insulin-like Androgenic Gland) in male Macrobrachium rosenbergii. This is the first time to use siRNA approach to silenced MrIAG in male M. rosenbergii. In the current study, the optimal injection dosage to achieve sex reversal is 0.5 μg/g body weight. After MrIAG silencing, the expression level of Dmrt11e, Dmrt99b, MRPINK, Mrr, Sxl1, and Sxl2 decreased significantly. As their long-term silencing effect of MrIAG, the dsRNA and siRNA approaches produce three and two individual neo-females, respectively. The neo-female has a wider brood chamber, ovipositing setae, and ovigerous setae, which is resembled normal female. After a long-term silencing with siRNA, most of the germ cells were arrested in spermatocytes stage, but the spermatocytes in control can further developed into spermatozoon. The seminiferous tubules are loosely arranged and the spermatocytes are more than spermatozoon in the 0.5 μg/g body weight treatment dose. This current study suggests a new path to obtain neo-females through siRNA silencing.

中文翻译：

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siRNA介导的MrIAG沉默诱导罗氏沼虾的性逆转。

胰岛素样雄性腺（IAG）基因在雄性甲壳动物中是众所周知的，并且是雄性性别分化和维持雄性特性的关键调节剂。的新雌性可通过沉默来制造MrIAG（罗氏沼虾胰岛素样雄腺）在雄性罗氏沼虾。这是首次使用siRNA方法沉默雄性罗氏沼虾的MrIAG。在当前的研究中，实现性逆转的最佳注射剂量为0.5μg/ g体重。后MrIAG沉默，的表达水平Dmrt11e，Dmrt99b，MRPINK，Mrr，Sxl1和Sxl2明显减少。作为MrIAG的长期沉默作用，dsRNA和siRNA方法分别产生了3个和2个独立的新女性。新女性具有较宽的巢腔，产卵的刚毛和刚毛的刚毛类似于正常的雌性。长期沉默后，大多数生殖细胞被阻滞在精母细胞阶段，但对照中的精母细胞可以进一步发育成精子。在0.5μg/ g体重的治疗剂量下，曲细精管松散地排列，并且精细胞比精子多。这项最新研究提出了通过siRNA沉默获得新女性的新途径。