Muga silk nanoparticles (MSNP) were synthesized using a microwave‐assisted radiolysis method. The effect of microwave on the Muga protein secondary structures was analyzed. The evolution of the secondary structure from random coils to the β‐sheets was determined by using FTIR, circular dichroism and X‐ray diffraction techniques. The results showed that Muga silk fibroin protein contained the primary structure in silk‐I state. When the protein was irradiated with microwave, nanoparticle synthesis was possible having silk‐II state imparting crystallinity. The silk nanoparticles were characterized by a particle size analyzer and found to be of ~240 nm in size. The optical properties of these nanoparticles were studied by UV–vis. spectroscopy and photoluminescence. For studying thermal properties, differential scanning calorimetry was performed that revealed early glass transition, which could be attributed to the presence of water and proteins. It also revealed that nanoparticles are thermally stable. Such studies are important for understanding more about the MSNP and would be beneficial for their further wide applications.

中文翻译：

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微波法合成穆加蚕丝纳米粒子的光谱分析

穆加丝绸纳米颗粒（MSNP）是使用微波辅助的放射分解方法合成的。分析了微波对Muga蛋白二级结构的影响。通过使用FTIR，圆二色性和X射线衍射技术确定了从无规卷曲到β折叠的二级结构的演变。结果表明，Muga丝素蛋白蛋白以Silk-I状态包含一级结构。当蛋白质用微波辐照时，纳米丝合成可能具有赋予结晶性的Silk-II状态。丝绸纳米颗粒通过粒度分析仪进行了表征，发现尺寸约为〜240 nm。这些纳米颗粒的光学性质通过紫外可见光谱进行了研究。光谱学和光致发光。为了研究热性能，进行差示扫描量热法，揭示了玻璃的早期转变，这可能归因于水和蛋白质的存在。还揭示了纳米颗粒是热稳定的。这样的研究对于进一步了解MSNP非常重要，并且对它们的进一步广泛应用也将是有益的。