Abstract

In order to study the effects of heat shock treatment on the distribution of rDNA contacts at the region possessing DUX genes inside chromosome 4 we used 4C approach. Our data indicate that the treatment removes the frequent rDNA contacts in this region. The recent data on involvement of superenhancers that are decorated by broad H3K27ac marks in the phase separation mechanisms and the previous data demonstrating that these broad marks are the favorite sites of rDNA contacts taken together with our data on sensitivity of the contacts to the heat shock treatment suggest that the phase separation mechanisms are involved in the reversible rDNA-mediated regulation of gene expression via the contacts.

中文翻译：

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rDNA簇与人类4号染色体中DUX基因的染色体间接触对热休克治疗非常敏感。

摘要

为了研究热休克处理对4号染色体内部具有DUX基因的区域rDNA接触分布的影响，我们使用了4C方法。我们的数据表明该治疗消除了该区域中频繁的rDNA接触。最新的有关超级增强剂参与H3K27ac标记参与相分离机制的数据，以及先前的数据表明这些广泛的标记是rDNA接触的最爱部位，以及我们对接触热敏处理敏感性的数据提示相分离机制涉及通过接触的可逆rDNA介导的基因表达调控。