Abstract

New recombinant carriers—modular nanotransporters (MNTs)—with N-terminal ligand module to the epidermal growth factor receptor (EGFR) were developed and characterized. Human epidermal growth factor (hEGF) and antibody-like protein Z₁₉₀₇ were used as a ligand module. We demonstrated that MNTs are able to internalize in a receptor-specific manner into the target cancer cells and to accumulate in the target cell nuclei. Conjugation of MNTs with the Auger electron emitter ¹¹¹In significantly enhanced the cytotoxic effect of ¹¹¹In on the target cells. It was found that the transfer of EGF from the C-terminus to the N-terminus of the MNT enhanced the proliferation of target cells, whereas the use of Z₁₉₀₇ did not have a similar effect.

中文翻译：

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新的重组载体特异性结合到表皮生长因子受体。

摘要

具有表皮生长因子受体（EGFR）的N端配体模块的新型重组载体-模块化纳米转运蛋白（MNT）。人类表皮生长因子（hEGF）和抗体样蛋白Z ₁₉₀₇被用作配体模块。我们证明，MNTs能够以受体特异性方式内化到靶癌细胞中并在靶细胞核中积累。MNT与俄歇电子发射器¹¹¹ In的结合显着增强了¹¹¹ In对靶细胞的细胞毒性作用。发现EGF从MNT的C末端转移到N末端增强了靶细胞的增殖，而使用Z ₁₉₀₇没有类似的作用。