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Electrochemically prepared poly(L-lysine) and 3-hydroxyphenylboronic acid composite as a conventional adhesion material for rice suspension cells
Electrochemistry Communications ( IF 5.4 ) Pub Date : 2020-04-27 , DOI: 10.1016/j.elecom.2020.106737
Mei Zeng , Tiean Zhou , Zhaohong Su , Weisong Pan

Poly(L-lysine) (PLL)-3-hydroxyphenylboronic acid (3-PBA) composite was prepared onto an Au electrode via cyclic voltammetry (CV), which was characterized by electrochemical and quartz crystal microbalance (QCM) techniques, and used to promote adhesion of plant cells. 3-PBA can interact with glycosyl compounds on the cell wall to form negatively charged covalent compounds while PLL increases the number of positively charged sites, which enhances the electrostatic interactions with the negatively charged cell wall, both promoting cell adhesion. The PLL-poly(3-PBA) composite was successfully modified on the Au electrode surface, as demonstrated by QCM and Fourier transform infrared spectrophotometry (FTIR). Moreover, we confirmed the synergistic effect of PLL and 3-PBA to adhere rice suspension cells using CV, electrochemical impedance spectroscopy (EIS), QCM and optical microscope observations. Finally, the dynamic process of rice cells’ adhesion to PLL-poly(3-PBA)/Au followed by salt stress treatment of NaCl was monitored in real-time by QCM. The cells softened at first then hardened under the action of 40 mM NaCl. This work presents a novel and conventional way to immobilize plant cells onto sensors, and has importance in the study of the structure, function and behavior of plants under various stresses at the cellular level.



中文翻译:

电化学制备的聚(L-赖氨酸)和3-羟基苯基硼酸复合材料作为水稻悬液细胞的常规粘附材料

通过循环伏安法(CV)在金电极上制备了聚(L-赖氨酸)(PLL)-3-羟基苯基硼酸(3-PBA)复合材料,通过电化学和石英晶体微天平(QCM)技术对其进行了表征,并用于促进植物细胞的粘附。3-PBA可以与细胞壁上的糖基化合物发生相互作用,从而形成带负电荷的共价化合物,而PLL增加了带正电荷的位点的数量,从而增强了与带负电荷的细胞壁的静电相互作用,均促进了细胞粘附。如QCM和傅里叶变换红外分光光度法(FTIR)所示,成功地在Au电极表面改性了PLL-poly(3-PBA)复合材料。此外,我们证实了PLL和3-PBA使用CV粘附水稻悬浮细胞的协同作用,电化学阻抗谱(EIS),QCM和光学显微镜观察。最后,通过QCM实时监测了水稻细胞对PLL-poly(3-PBA)/ Au的粘附过程以及随后的NaCl盐胁迫处理的过程。细胞首先变软,然后在40 mM NaCl的作用下变硬。这项工作提出了一种新颖的常规方法,将植物细胞固定在传感器上,并且在研究细胞水平下各种胁迫下植物的结构,功能和行为方面具有重要意义。

更新日期:2020-04-27
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