Models for enteric neuropathies, in which intestinal nerves are absent or injured, are required to evaluate possible cell therapies. However, existing options, including transgenic mice, are variable and fragile. Here immunocompromised mice were implanted with human pluripotent stem cell-derived tissue-engineered small intestine 10 weeks prior to a second survival surgery in which enteric nervous system precursor cells, or saline controls, were injected into the human intestinal organoid-derived tissue-engineered small intestine and analyzed 4 weeks later. Human intestinal organoid-derived tissue-engineered small intestine implants injected with saline as controls illustrated formation of intestinal epithelium and mesenchyme without an enteric nervous system. Second surgical introduction of human pluripotent stem cell-generated enteric nervous system precursors into developing human intestinal organoid-derived tissue-engineered small intestine implants resulted in proliferative migratory neuronal and glial cells, including multiple neuronal subtypes, and demonstrated function in contractility assays.

中文翻译：

---

诱导的多能干细胞衍生的肠神经cells细胞在人类神经节组织工程化的肠道中重新形成肠道神经系统的关键成分。

需要评估肠神经缺失或肠神经受损的肠道神经病模型，以评估可能的细胞疗法。然而，包括转基因小鼠在内的现有选择是可变的且脆弱的。在这里，免疫功能低下的小鼠在第二次生存手术前10周植入了人类多能干细胞衍生的组织工程小肠，在该手术中，将肠道神经系统前体细胞或盐水对照注射到了人类肠道类器官衍生的组织工程小肠中。肠道，并在4周后进行分析。注射盐水作为对照的人肠类器官衍生的组织工程小肠植入物说明了肠上皮和间充质的形成，而没有肠神经系统。