Eugenia punicifolia (Kunth) D. C. (Myrtaceae) has been showing interesting biological activities in the literature which was correlated to its phenolic compounds. In the sense of a better recovering of phenolics with the best antioxidant and antiproliferative activities, an extraction, based on multivariate analytical approach, was developed from E. punicifolia leaves. The different extractor solvents (ethanol, methanol and water) and their binary and ternary combinations were evaluated using a simplex-centroid mixture design and surface response methodology. The optimized crude extracts were investigated for phenol and flavonoid content and compared to their antioxidant (EC50) and antiproliferative properties against HEp-2 (cell line derived from the oropharyngeal carcinoma) and mononuclear viability cells. Ethanolic extracts showed the best phenolic content with the highest antioxidant activity and moderated activity antiproliferative to HEp-2. ESI-QTOF-MS revealed the presence of quercetin and myricetin derivatives, which was correlated to activities tested. Then, simplex-centroid design allowed us to correlate the Eugenia punicifolia biological activities with the extracts obtained from solvent different polarity mixtures.

中文翻译：

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使用单纯形质心设计优化番泻叶（Kingth）番石榴DC叶片的提取，重点在于提取具有抗氧化和抗增殖活性的酚类。

Eugenia punicifolia（Kunth）DC（Myrtaceae）在文献中已显示出有趣的生物学活性，这与其酚类化合物有关。为了更好地回收具有最佳抗氧化剂和抗增殖活性的酚类物质，从多元分析方法中提取了一种从E. punicifolia叶片中提取的提取物。使用单纯形-质心混合物设计和表面响应方法，评估了不同的萃取溶剂（乙醇，甲醇和水）及其二元和三元组合。研究了优化的粗提物的酚和类黄酮含量，并将其与抗氧化剂（EC50）和对HEp-2（来自口咽癌的细胞系）和单核存活细胞的抗增殖特性进行了比较。乙醇提取物显示出最佳的酚含量，具有最高的抗氧化活性和适度的抗HEp-2增生活性。ESI-QTOF-MS揭示了槲皮素和杨梅素衍生物的存在，这与测试的活性有关。然后，单纯形-质心设计使我们能够将Eugenia punicifolia的生物活性与从溶剂不同极性混合物中获得的提取物相关联。