Arsenic(As), antimony(Sb) and selenium(Se) are redox-sensitive trace elements in environment. Up to date large gap in knowledge still exists with respect to their toxicity, bioavailability, adsorption and mobility due to lack of robust analytical methods allowing simultaneous speciation analysis of various species. Despite the publication of numerous analytical methods concerning As, Sb and/or Se, people seem still at a loss how to decide a proper method. In addition, previous reviews of analytical methods of these three elements concern mainly chromatography based methods, and generally only individual element was considered. We overviewed the state-of-the-art of the speciation analysis methods for the three elements, including chromatography-based methods and non-chromatographic methods, with a focus on simultaneous speciation analysis of them. All kinds of methods were elucidated from method principle in combination with specific properties of each species. This manuscript aims to provide a comprehensive understanding of speciation analysis methods, especially simultaneous speciation analysis methods for As, Sb and Se, which is also helpful for those who are interested in speciation analysis of other multi-species trace elements.

砷（As），锑（Sb）和硒（Se）是环境中对氧化还原敏感的微量元素。迄今为止，由于缺乏可靠的分析方法，无法同时进行各种物种的形态分析，因此在毒性，生物利用度，吸附和迁移性方面仍存在很大的知识空白。尽管已经发表了许多有关As，Sb和/或Se的分析方法，但是人们似乎仍然茫然如何确定合适的方法。另外，先前对这三种元素的分析方法的评论主要涉及基于色谱的方法，并且通常仅考虑单个元素。我们概述了这三种元素的最新形态分析方法，包括基于色谱的方法和非色谱方法，重点是对它们的同时形态分析。从方法原理出发，结合每种物种的具体特性，阐明了各种方法。该手稿旨在提供对形态分析方法的全面理解，尤其是对As，Sb和Se的同时形态分析方法，这也对那些对其他多物种痕量元素的形态分析感兴趣的人有所帮助。