In the present study, ultrasound-assisted extraction of water-soluble polysaccharides from the Acanthus ilicifolius was optimized by using one-factor-at-a-time and Box–Behnken designs. A maximum polysaccharide yield of 2.46% was obtained under the optimized conditions of ultrasonic power of 150 W, temperature of 53 °C, extraction time of 50 min, and liquid to solid ratio of 35 mL/g. Results of UV, FTIR and molecular weights indicated that there were significant differences in structures of polysaccharides extracted with ultrasound assistance (UAE-ACI) and hot-water extraction (HWE-ACI) including monosaccharide compositions and ratios, and molecular weight distributions. Further analysis showed that UAE-ACI had a higher antioxidant activity in vitro than that of HWE-ACI possibly due to lower molecular weight and higher content of phenolics and protein fractions.

中文翻译：

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超声波辅助提取i叶多糖的抗氧化活性

在本研究中，超声辅助的水溶性多糖提取从老鼠簕通过一次使用一个因子和Box–Behnken设计进行了优化。在150 W的超声波功率，53°C的温度，50分钟的提取时间和35 mL / g的液固比的优化条件下，最大多糖收率为2.46％。UV，FTIR和分子量的结果表明，超声辅助提取的多糖（UAE-ACI）和热水提取的多糖（HWE-ACI）的结构存在显着差异，包括单糖的组成和比例以及分子量分布。进一步的分析表明，UAE-ACI在体外具有比HWE-ACI更高的抗氧化活性，这可能是由于分子量较低，酚类和蛋白质组分含量较高。