BACKGROUND Obesity and osteoporosis are two chronic conditions that have been increasing in prevalence. Menopausal transition years place women at high risk for visceral obesity as well as osteoporosis. This study was carried out to elucidate the effect of visceral adiposity on ovariectomy-induced osteoporosis in rats. METHODS We studied female Wistar rats aged 12-14 months, divided into four groups: a) Sham-operated control (SHAM) rats (n = 12), rats were fed a control diet (59% of food intake from carbohydrates, 7% from fat, 21% from protein, 13% from minerals and ash) for 12 weeks, b) High fat diet-fed control (HFD) group (n = 9), rats were fed a high fat diet (49% of food intake from carbohydrates, 17% from fat, 21% from protein, 13% from minerals and ash)for 12 weeks, c) Ovariectomized (OVX) rats (n = 14), rats were fed a control diet as SHAM rats, d) High fat diet- fed ovariectomized (OVX- HFD) rats (n = 13), rats were fed a high fat diet as HFD group. At the end of the experiment, blood samples were collected for calcium, phosphorus, and alkaline phosphatase (ALP) assays. Unilateral left perirenal fats were surgically removed and weighed. Specimens from right perirenal fats and tibia were isolated and processed for histological examination. Histomorphometric analysis of the tibia and visceral adipose tissue was also performed. RESULTS OVX, HFD, and OVX-HFD rats showed a significant increase in relative visceral fat weight, and plasma ALP, and a significant decrease in plasma calcium, and phosphorus levels compared to SHAM rats. Light microscopic examination of the tibia of the OVX rats revealed a significant decrease in the cortical bone thickness (CBT) and the trabecular bone thickness (TBT), and a significant increase in bone marrow adipose tissue compared to SHAM rats. In addition, there was a significant increase in the osteoclast number, and a significant decrease in the osteoblast number. The changes in bone marrow adipose tissue as well as osteoclast number were further accentuated in OVX-HFD groups. CONCLUSIONS Visceral obesity played a crucial role in the development of osteoporosis in ovariectomized rats through effects that might involve both osteoblasts and osteoclasts.

中文翻译：

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在卵巢切除大鼠中作为绝经后骨质疏松症模型的骨质疏松症发病机理的新方法。

背景技术肥胖症和骨质疏松症是两个流行率不断增加的慢性病。绝经过渡年使妇女有内脏肥胖和骨质疏松症的高风险。进行这项研究以阐明内脏肥胖对大鼠卵巢切除术引起的骨质疏松的影响。方法我们研究了12-14个月大的Wistar雌性大鼠，分为四组：a）假手术对照（SHAM）大鼠（n = 12），大鼠接受对照饮食（59％的碳水化合物摄入食物，7％的食物摄入）脂肪，蛋白质占21％，矿物质和灰分占13％），持续12周； b）高脂饮食喂养对照（HFD）组（n = 9），大鼠接受高脂饮食（食物摄入量的49％） 12周内，从碳水化合物中提取，从脂肪中提取17％，从蛋白质中提取21％，从矿物质和粉尘中提取13％）c）去卵巢（OVX）大鼠（n = 14），给大鼠喂食作为对照饮食的SHAM大鼠。d）给高脂饮食喂食去卵巢（OVX-HFD）大鼠（n = 13），给大鼠喂食高脂饮食作为HFD组。实验结束时，收集血液样本进行钙，磷和碱性磷酸酶（ALP）分析。手术切除单侧左肾周围脂肪并称重。分离出来自右肾周围脂肪和胫骨的标本并进行处理以进行组织学检查。还对胫骨和内脏脂肪组织进行了组织形态分析。结果与SHAM大鼠相比，OVX，HFD和OVX-HFD大鼠相对内脏脂肪重量和血浆ALP显着增加，血浆钙和磷水平显着下降。与SHAM大鼠相比，OVX大鼠胫骨的光学显微镜检查显示皮质骨厚度（CBT）和小梁骨厚度（TBT）显着减少，而骨髓脂肪组织显着增加。另外，破骨细胞数目显着增加，而成骨细胞数目显着减少。OVX-HFD组的骨髓脂肪组织和破骨细胞数量的变化更加明显。结论内脏肥胖通过可能涉及成骨细胞和破骨细胞的作用在去卵巢大鼠的骨质疏松发展中起着关键作用。破骨细胞数量显着增加，成骨细胞数量显着减少。OVX-HFD组的骨髓脂肪组织和破骨细胞数量的变化更加明显。结论内脏肥胖通过可能涉及成骨细胞和破骨细胞的作用在去卵巢大鼠的骨质疏松发展中起着关键作用。破骨细胞数量显着增加，成骨细胞数量显着减少。OVX-HFD组的骨髓脂肪组织和破骨细胞数目的变化进一步加重。结论内脏肥胖通过可能涉及成骨细胞和破骨细胞的作用在去卵巢大鼠的骨质疏松发展中起着关键作用。