Invasive fungal infections are responsible for a significant disease burden worldwide. Drugs to treat these infections are limited to only four unique classes, and despite these available treatments, mortality rates remain unacceptably high. In this review, we will discuss antifungal drug screening and how the approach to identifying novel compounds needs move away from traditional growth-based assays in order to meet the demand for new drugs. We highlight specific examples of creative screening strategies that increase the likelihood of identifying compounds with desired activities and provide perspective to inspire development of novel screens for the identification of first-in-class antifungals.

中文翻译：

---

抗真菌药物筛选：跳出框框思考，以识别新型抗真菌支架。

侵袭性真菌感染是全球范围内重大疾病负担的原因。治疗这些感染的药物仅限于四个独特的类别，尽管有这些可用的治疗方法，死亡率仍然高得令人无法接受。在这篇综述中，我们将讨论抗真菌药物的筛选以及鉴定新化合物的方法如何摆脱传统的基于生长的分析方法，从而满足对新药的需求。我们重点介绍了创造性筛选策略的具体示例，这些示例增加了鉴定具有所需活性的化合物的可能性，并提供了启发性灵感，以开发新颖的筛查技术来鉴定一流的抗真菌药。