Cerebral edema after brain surgery remains a life-threatening complication in the clinic. For a better operating field view, superior petrosal vein (SPV) can be easily damaged during neurosurgery. SPV sacrifice may sometimes be inevitable in clinic. However, the safety of SPV sacrifice is still a controversial question. Whether petrosal vein injury has an effect on cerebral edema after brain surgery is still unknown. In this study, rabbits were divided into two groups. The rabbits in the surgery group underwent petrosal vein sacrifice. The control group was subjected to sham surgery. Cerebellum and brain stem tissues were collected at 4 h, 8 h, 12 h, 24 h, 48 h and 72 h post-surgery. The superoxidase dismutase (SOD) activity and expression of malondialdehyde (MDA) were tested in the collected samples. Quantitiative real time polymerase chain reaction and immunohistochemistry were used to detect the mRNA and protein levels, respectively, of aquaporin 4 (AQP4) in the tissue samples. Compared to the control sham group, the activity of SOD and MDA expression in cerebellum was decreased and increased, respectively, at 4 h, 8 h, 12 h and 24 h post-, surgery The SOD activity and expression of MDA in brain stem was decreased and increased, respectively, only in 4 h after surgery, compared with control group. The mRNA and protein levels of AQP4 were increased in cerebellum at 4 h, 8 h, 12 h and 24 h after surgery, but in the brain stem, the levels were increased only at 4 h after surgery compared with sham group. Our results thus show that SPV sacrifice influences oxidative stress and the expression of AQP4 in cerebellum and brain stem of rabbits; highlighting the importance of protecting the petrosal vein during neurosurgery.

中文翻译：

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破坏岩静脉对兔小脑和脑干SOD活性、MDA水平和AQP4水平的影响

脑部手术后的脑水肿仍然是临床上危及生命的并发症。为了获得更好的手术视野，在神经外科手术过程中，岩上静脉 (SPV) 很容易受损。SPV 牺牲有时在临床上是不可避免的。然而，SPV牺牲的安全性仍然是一个有争议的问题。岩静脉损伤是否对脑手术后脑水肿有影响尚不清楚。在这项研究中，兔子被分为两组。手术组兔进行岩静脉处死。对照组进行假手术。在术后 4 小时、8 小时、12 小时、24 小时、48 小时和 72 小时收集小脑和脑干组织。在收集的样品中测试了超氧化物酶歧化酶（SOD）活性和丙二醛（MDA）的表达。定量实时聚合酶链反应和免疫组织化学分别用于检测组织样本中水通道蛋白 4 (AQP4) 的 mRNA 和蛋白质水平。与对照组相比，手术后 4 h、8 h、12 h 和 24 h 小脑 SOD 活性和 MDA 表达量分别降低和升高。仅在术后 4 h 分别与对照组相比减少和增加。与假手术组相比，术后4、8、12、24小时小脑AQP4的mRNA和蛋白水平升高，而脑干仅在术后4小时升高。因此，我们的结果表明，SPV 处死会影响氧化应激和兔小脑和脑干中 AQP4 的表达；