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Impacts of different synthetic polymers on vitrification of ovarian tissue
Cryobiology ( IF 2.7 ) Pub Date : 2020-06-01 , DOI: 10.1016/j.cryobiol.2020.04.007
Mohammad Hamed Shahsavari 1 , Kele Amaral Alves 2 , Benner Geraldo Alves 3 , Laritza Ferreira de Lima 3 , Diego Alberto Montano Vizcarra 3 , Deysi Juana Dipaz Berrocal 3 , Luciana Mascena Silva 3 , Yago Pinto da Silva 3 , Mary B Zelinski 4 , José Ricardo de Figueiredo 3 , Gholamali Moghaddam 1 , Ana Paula Ribeiro Rodrigues 3
Affiliation  

Type and concentration of cryoprotective agents (CPAs) are important factors which influence the likelihood of a successful ovarian tissue vitrification outcome. In an attempt to address this factor, the present study was conducted to evaluate the impacts of different synthetic polymers (Supercool X-1000, Supercool Z-1000 and PVP K-12) on vitrification of bovine ovarian tissue. From each ovarian pair, fragments were recovered and immediately fixed for analysis (fresh control) or submitted to vitrification, either or not followed by in vitro culture for one or five days. Vitrification was performed using the ovarian tissue cryosystem (OTC) system. The ovarian tissues were intended for histological and viability analysis [Reactive oxygen species (ROS) production and degenerate cells assay (Ethidium homodimer-1)], as well as immunolocalization of AQP3 and AQP9 were measured. The results showed that during almost all the periods after warming, in treatment groups which contain polymer (X-1000, Z-1000 and PVP), the percentage of morphologically normal follicles was the highest in the X-1000 samples. Furthermore, post-thawed X-1000 group revealed stronger labeling for AQP9 in primordial and transitional follicles, when compared with others. However, morphology after cryopreservation did not correlate with follicle viability and function where the levels of degeneration and tissue damage of PVP K-12 group were lower in comparison with X-1000 group and only in PVP K-12 group, ROS level was similar to that of the fresh control group. We believe that in addition to permeating CPAs, the addition of one (Supercool X-1000) or maybe a combination (Supercool X-1000 and PVP K-12) of non-permeating polymers could be useful to improve the outcome for vitrified bovine ovarian tissue.

中文翻译:

不同合成聚合物对卵巢组织玻璃化的影响

冷冻保护剂 (CPA) 的类型和浓度是影响卵巢组织玻璃化成功可能性的重要因素。为了解决这个问题,本研究旨在评估不同合成聚合物(Supercool X-1000、Supercool Z-1000 和 PVP K-12)对牛卵巢组织玻璃化的影响。从每个卵巢对中,回收碎片并立即固定用于分析(新鲜对照)或进行玻璃化冷冻,然后进行 1 天或 5 天的体外培养。使用卵巢组织冷冻系统 (OTC) 系统进行玻璃化冷冻。卵巢组织用于组织学和活力分析 [活性氧 (ROS) 产生和退化细胞测定 (Ethidium homodimer-1)],以及 AQP3 和 AQP9 的免疫定位被测量。结果表明,在加热后的几乎所有时间段内,在含有聚合物(X-1000、Z-1000 和 PVP)的治疗组中,形态正常的卵泡百分比在 X-1000 样品中最高。此外,与其他组相比,解冻后的 X-1000 组在原始和过渡卵泡中显示出更强的 AQP9 标记。然而,冷冻保存后的形态与卵泡活力和功能无关,PVP K-12 组的变性和组织损伤水平低于 X-1000 组,仅 PVP K-12 组的 ROS 水平与新鲜对照组。我们认为,除了渗透 CPA,
更新日期:2020-06-01
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