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MicroRNA-21 as a regulator of human cumulus cell viability and its potential influence on the developmental potential of the oocyte.
Biology of Reproduction ( IF 3.6 ) Pub Date : 2020-04-25 , DOI: 10.1093/biolre/ioaa058
Alison F Bartolucci 1, 2 , Tracy Uliasz 3 , John J Peluso 1, 3
Affiliation  

MicroRNA-21 is expressed in bovine, murine, and human cumulus cells with its expression in murine and bovine cumulus cells correlated with oocyte developmental potential. The aim of this study was to assess the relationship between cumulus cell MIR-21 and human oocyte developmental potential. These studies revealed that both the immature and mature forms of MicroRNA-21 (MIR-21-5p) were elevated in cumulus cells of oocytes that developed into blastocysts compared to cumulus cells of oocytes that arrested prior to blastocyst formation. This increase in MicroRNA-21 was observed regardless of whether the oocytes developed into euploid or aneuploid blastocysts. Moreover, MIR-21-5p levels in cumulus cells surrounding oocytes that either failed to mature or matured to metaphase II but failed to fertilize, were ≈50% less than the MIR-21-5p levels associated with oocytes that arrested prior to blastocyst formation. Why cumulus cells associated with oocytes of reduced developmental potential expressed less MIR-21-5p is unknown. It is unlikely due to reduced expression of either the receptors of growth differentiation factor 9 or rosha Ribonuclease III (DROSHA) and Dicer Ribonuclease III (DICER) which sequentially promote the conversion of immature forms of MicroRNA-21 to mature MicroRNA-21. Furthermore, cultured cumulus cells treated with a MIR-21-5p inhibitor had an increase in apoptosis and a corresponding increase in the expression of PTEN, a gene known to inhibit the AKT-dependent survival pathway in cumulus cells. These studies provide evidence for a role of MicroRNA-21 in human cumulus cells that influences the developmental potential of human oocytes.

中文翻译:

MicroRNA-21 作为人类卵丘细胞活力的调节剂及其对卵母细胞发育潜力的潜在影响。

MicroRNA-21 在牛、鼠和人卵丘细胞中表达,其在鼠和牛卵丘细胞中的表达与卵母细胞发育潜力相关。本研究的目的是评估卵丘细胞MIR-21与人类卵母细胞发育潜力之间的关系。这些研究表明,与在囊胚形成之前停滞的卵母细胞的卵丘细胞相比,发育成囊胚的卵母细胞的卵丘细胞中未成熟和成熟形式的 MicroRNA-21 ( MIR-21-5p ) 均升高。无论卵母细胞发育成整倍体还是非整倍体囊胚,都观察到了 MicroRNA-21 的这种增加。此外,MIR-21-5p未能成熟或成熟到中期 II 但未能受精的卵母细胞周围卵丘细胞中的卵丘细胞水平比与在囊胚形成之前停滞的卵母细胞相关的MIR-21-5p水平低约 50% 。为什么与发育潜能降低的卵母细胞相关的卵丘细胞表达较少的MIR-21-5p尚不清楚。这是不可能的,由于减少的生长分化因子9或rosha核糖核酸酶III(无论是受体的表达DROSHA)和切丁酶核糖核酸酶III(DICER),其顺序地促进的微小RNA-21的不成熟的形式转化为成熟的微小RNA-21。此外,用MIR-21-5p处理培养的卵丘细胞抑制剂具有细胞凋亡增加和PTEN表达的相应增加,PTEN是一种已知抑制卵丘细胞中 AKT 依赖性存活途径的基因。这些研究为 MicroRNA-21 在影响人类卵母细胞发育潜力的人类卵丘细胞中的作用提供了证据。
更新日期:2020-06-24
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