As obesity incidence is alarmingly rising among young individuals, we aimed to characterize an experimental model of this situation, considering the similarity between human and porcine physiology. For this reason, we fed prepubertal (63 days old) Duroc breed females (n=21) either with a standard growth diet (3800 kcal/day) or one with a high-calorie content (5200 kcal/day) during 70 days. Computerized tomography, mass-spectrometry-based metabolomics and lipidomics, as well as peripheral blood mononuclear cell transcriptomics, were applied to define traits linked to high-calorie intake. Samples from a human cohort confirmed potential lipidomic markers. Compared to those fed a standard growth diet, pigs fed a high-calorie diet showed an increased weight gain (13%), much higher adiposity (53%), hypertriacylglyceridemia and hypercholesterolemia in parallel to insulin resistance. This diet induced marked changes in the circulating lipidome, particularly in phosphatidylethanolamine-type molecules. Also, circulating specific diacylglycerol and monoacylglycerol contents correlated with visceral fat and intrahepatic triacylglycerol concentrations. Specific lipids associated with obesity in swine (mainly belonging to glycerophospholipid, triacylglyceride and sterol classes) were also linked with obesity traits in the human cohort, reinforcing the usefulness of the chosen approach. Interestingly, no overt inflammation in plasma or adipose tissue was evident in this model. The presented model is useful as a preclinical surrogate of prepubertal obesity in order to ascertain the pathophysiology interactions between energy intake and obesity development.

由于肥胖症的发病率在年轻人中呈惊人的上升趋势，因此，我们考虑到人与猪生理之间的相似性，旨在表征这种情况的实验模型。因此，我们喂养了青春期前（63天大）杜洛克种雌性（n= 21）在70天内使用标准生长饮食（3800 kcal /天）或高热量饮食（5200 kcal /天）。计算机断层扫描，基于质谱的代谢组学和脂质组学以及外周血单核细胞转录组学被用于定义与高热量摄入相关的特征。来自人类队列的样品证实了潜在的脂质组学标记。与饲喂标准生长日粮的猪相比，饲喂高热量日粮的猪增重（13％），肥胖率（53％）高得多，高三酰甘油血症和高胆固醇血症与胰岛素抵抗平行。这种饮食在循环脂质组中引起显着变化，尤其是磷脂酰乙醇胺型分子。也，循环中特定的二酰基甘油和单酰基甘油含量与内脏脂肪和肝内三酰基甘油浓度相关。与猪肥胖相关的特定脂质（主要属于甘油磷脂，三酰甘油和固醇类）也与人类队列中的肥胖特征相关，从而增强了所选方法的实用性。有趣的是，在该模型中没有明显的血浆或脂肪组织炎症。所提出的模型可用作青春期前肥胖的临床前替代品，以便确定能量摄入与肥胖发展之间的病理生理学相互作用。甘油三酸酯和甾醇类）也与人类队列中的肥胖性状有关，从而增强了所选方法的实用性。有趣的是，在该模型中，血浆或脂肪组织中没有明显的炎症。所提出的模型可用作青春期前肥胖的临床前替代品，以便确定能量摄入与肥胖发展之间的病理生理学相互作用。甘油三酸酯和固醇类）也与人类队列中的肥胖性状有关，从而增强了所选方法的实用性。有趣的是，在该模型中，血浆或脂肪组织中没有明显的炎症。所提出的模型可用作青春期前肥胖的临床前替代品，以便确定能量摄入与肥胖发展之间的病理生理学相互作用。