Mercury (Hg) is a heavy metal with high toxicity and easy migration; it can be enriched through the food chain, and cause serious threats to the natural environment and human health. So, the development of a method that can be used to detect mercury ions (Hg²⁺) in the environment, in cells, and in organisms is very important. Here, a new 7‐hydroxycoumarin‐derived carbonothioate‐based probe (CC‐Hg) was designed and synthesized for detection of Hg²⁺. After addition of Hg²⁺, a large fluorescence enhancement was observed due to the formation of 7‐hydroxyl, which reinforced the intramolecular charge transfer process. The CC‐Hg probe had good water solubility and selectivity. Moreover, the probe was able to detect Hg²⁺ quantitatively over the concentration range 0–2 μM and with a detection limit of 7.9 nM. Importantly, we successfully applied the probe to detect Hg²⁺ in water samples, in living cells, and in zebrafish. The experimental results demonstrated its potential value in practical applications.

中文翻译：

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基于香豆素的Hg2 +荧光探针及其在活细胞和斑马鱼中的应用。

汞（Hg）是一种重金属，具有高毒性和易迁移的特性。它可以通过食物链得到丰富，并严重威胁自然环境和人类健康。因此，开发一种可用于检测环境，细胞和生物体中汞离子（Hg ²⁺）的方法非常重要。在这里，设计并合成了一种新的基于7-羟基香豆素的硫代碳酸盐探针（CC-Hg），用于检测Hg ²⁺。加入Hg ^2+后，由于7-羟基的形成，荧光增强了，这增强了分子内电荷转移的过程。在CC-汞柱探针具有良好的水溶性和选择性。此外，该探针能够在0–2μM的浓度范围内定量检测Hg ²⁺，检测极限为7.9 nM。重要的是，我们成功地将探针应用于水样，活细胞和斑马鱼中的Hg ²⁺检测。实验结果证明了其在实际应用中的潜在价值。