Two different genotypes of Pinus massoniana seedlings (aluminum-resistant FJ5 and aluminum-sensitive GD20) were used, the effects of different exogenous malic acid (0.00, 0.01, 0.02, 0.04, 0.08, 0.16 mmol·L-1) on the growth attributes of P. massoniana seedlings treated by the Al3+ concentration of 0.8 mmol·L-1 were studied, to provide a basis for the growth in acidified soil. In our experiment, the seedling growth was inhibited by Al3+ treatment. After treatment with a low concentration of exogenous malic acid, the activities of antioxidant enzymes in leaves were enhanced, the concentrations of hydrogen peroxide (H2O2), superoxide (O2-·), malondialdehyde (MDA) and osmotic adjustment substances were reduced accordingly. GD20 exhibited more severe changes compared with FJ5. The larger ones of the contribution rates of the indices in principal component analysis were H2O2, Glutathione Reductase (GR). These results indicated that Al3+ with high concentration inhibits the growth of P. massoniana. Malic acid could effectively alleviate the toxicity, and the mitigation effect on the aluminum-sensitive species, which genotype is more sensitive to the response of Al3+ toxicity, was more effective than that on the aluminum-resistant. How to select and cultivate more resistant species, by using the main parameter (H2O2 and GR), is worthy in further study.

中文翻译：

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外源苹果酸对减轻马尾松铝毒的影响。

使用两种不同基因型的马尾松幼苗（耐铝FJ5和铝敏感GD20），不同外源苹果酸（0.00、0.01、0.02、0.04、0.08、0.16mmol·L-1）对生长特性的影响研究了Al3 +浓度为0.8mmol·L-1对马尾松幼苗的酸化处理，为酸化土壤的生长提供了依据。在我们的实验中，Al3 +处理抑制了幼苗的生长。低浓度的外源苹果酸处理后，叶片中抗氧化酶的活性增强，过氧化氢（H2O2），超氧化物（O2-·），丙二醛（MDA）和渗透调节物质的浓度相应降低。与FJ5相比，GD20表现出更严重的变化。主成分分析中该指标的较大贡献率是H2O2，谷胱甘肽还原酶（GR）。这些结果表明高浓度的Al 3+抑制了马尾假单胞菌的生长。苹果酸可以有效地减轻毒性，对铝敏感物种的缓解基因型比对铝敏感的物种更有效。如何通过使用主要参数（H2O2和GR）选择和培育更多抗性物种，值得进一步研究。哪一个基因型对Al3 +毒性反应更敏感，比耐铝性更有效。如何通过使用主要参数（H2O2和GR）选择和培育更多抗性物种，值得进一步研究。哪一个基因型对Al3 +毒性反应更敏感，比耐铝性更有效。如何通过使用主要参数（H2O2和GR）选择和培育更多抗性物种，值得进一步研究。