PURPOSE The aim of this study was to compare the levels of hyperglycosylated human chorionic gonadotropin (hCG-H) secreted from balanced and unbalanced human embryos. METHODS Single-step culture media samples from 155 good quality embryos, derived from 90 good prognosis patients undergoing intracytoplasmic sperm injection (ICSI), were collected on the fifth day of embryo cultivation. All embryos were tested by next-generation sequencing (NGS) technique. The hCG-H levels in the culture media were evaluated by ELISA kit (Cusabio Biotech, CBS-E15803h) according to the manufacturer's instructions. Statistical analysis was performed using SPSS v.21 (IBM Corp., Armonk, NY, USA). RESULTS The NGS analysis revealed that 36% of the embryos (n = 56) were balanced, and 64% of the embryos were unbalanced (n = 99). The presence of hCG-H was confirmed in all embryo culture media samples but was absent in the negative control. In addition, hCG-H concentration was significantly higher in the culture media from unbalanced embryos compared with the balanced ones (0.72 ± 0.30 mIU/ml vs. 0.62 ± 0.12 mIU/ml, p = 0.02, respectively). Furthermore, the mean levels of hCG-H were significantly increased in the samples from embryos with multiple abnormalities. Finally, the highest levels of hCG-H were expressed from embryos with monosomy of chromosome 11 (1.28 ± 0.04 mIU/ml) and those with trisomies of chromosomes 21 (2.23 mIU/ml) and 4 (1.02 ± 0.35 mIU/ml). CONCLUSION Our results suggest that chromosomal aberrations in human embryos are associated with an increased secretion of hCG-H. However, hCG-H concentration in embryo culture media as a single biomarker is not sufficient for an accurate selection of balanced embryos.

中文翻译：

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不平衡的人类胚胎比平衡的人类胚胎分泌更多的高糖基化人绒毛膜促性腺激素（hCG-H）。

目的 本研究的目的是比较平衡和不平衡人类胚胎分泌的高糖基化人绒毛膜促性腺激素 (hCG-H) 水平。方法 在胚胎培养的第五天，从 90 名接受胞浆内单精子注射 (ICSI) 的预后良好的患者中收集了 155 个优质胚胎的一步培养基样本。所有胚胎均通过下一代测序（NGS）技术进行测试。根据制造商的说明，通过 ELISA 试剂盒（Cusabio Biotech，CBS-E15803h）评估培养基中的 hCG-H 水平。使用 SPSS v.21（IBM Corp.，Armonk，NY，USA）进行统计分析。结果 NGS 分析显示，36% 的胚胎 (n = 56) 是平衡的，64% 的胚胎是不平衡的 (n = 99)。在所有胚胎培养基样品中都证实了 hCG-H 的存在，但在阴性对照中不存在。此外，与平衡胚胎相比，不平衡胚胎的培养基中 hCG-H 浓度显着较高（分别为 0.72 ± 0.30 mIU/ml 与 0.62 ± 0.12 mIU/ml，p = 0.02）。此外，来自具有多种异常的胚胎的样本中 hCG-H 的平均水平显着增加。最后，最高水平的 hCG-H 表达来自具有 11 号染色体单体 (1.28 ± 0.04 mIU/ml) 的胚胎以及具有 21 号染色体三体性 (2.23 mIU/ml) 和 4 号染色体 (1.02 ± 0.35 mIU/ml) 的胚胎。结论 我们的结果表明，人类胚胎中的染色体畸变与 hCG-H 分泌增加有关。然而，胚胎培养基中的 hCG-H 浓度作为单一生物标志物不足以准确选择平衡胚胎。