Meat originating from roe, deer and especially chamois is expensive. Normally, such delicacies are provided directly by hunters and exclusively during the hunting season. In some cases, either to achieve higher profit or to enable total availability over a longer period, producers may keep only a low proportion of such meat in mixtures or even replace it completely by a less pricey meat product. Food control laboratories need sensitive, reliable, fast and easily applicable laboratory methods to detect such fraudulent practices and confirm species authenticity. We therefore developed a real-time PCR pentaplex using proven primers to detect simultaneously the presence of expensive species (roe, deer and chamois) and the potentially added meat from cheaper farm animals like pork and beef. In this context, we present the validation data for the quantitative determination of all five species in a multiplex real-time PCR. The measurement uncertainty was estimated to be 20%. A ring trial showed the robustness and practicability of this system. Market samples were analysed as well showing the applicability of this system to real-life samples.

中文翻译：

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多重实时PCR用于检测和定量羚羊、,、鹿，猪肉和牛肉中的DNA

来自ro子，鹿尤其是羚羊的肉价格昂贵。通常，这些美味佳肴是由猎人直接提供的，并且仅在狩猎季节提供。在某些情况下，为了获得更高的利润或在更长的时间内实现总供应量，生产商可能只将这种肉的一部分保留在混合物中，甚至完全用价廉的肉产品代替。食品控制实验室需要灵敏，可靠，快速且易于应用的实验室方法来检测此类欺诈行为并确认物种的真实性。因此，我们开发了一种实时PCR五重体，使用经过验证的引物来同时检测昂贵物种（,、鹿和羚羊）的存在以及可能从便宜的农场动物（如猪肉和牛肉）中添加的肉。在这种情况下，我们提供了用于实时定量PCR中所有5种物种定量测定的验证数据。测量不确定度估计为20％。环形试验显示了该系统的鲁棒性和实用性。对市场样本也进行了分析，显示了该系统对实际样本的适用性。