This study proposes the treatment and valorization of denim textile effluents through a fermentative hydrogen production process. Also, the study presents the decolorizing capabilities of bacterial and fungal isolates obtained from the fermented textile effluents. The maximum hydrogen production rate was 0.23 L H 2 /L-d, achieving at the same time color removal. A total of thirty-five bacteria and one fungal isolate were obtained from the fermented effluents and screened for their abilities to decolorize indigo dye, used as a model molecule. From them, isolates identified as Bacillus BT5, Bacillus BT9, Lactobacillus BT20, Lysinibacillus BT32, and Aspergillus H1T showed notable decolorizing capacities. Lactobacillus BT20 reached 90% of decolorization using glucose as co-substrate after 11 days of incubation producing colorless metabolites. Bacillus BT9 was able to utilize the indigo dye as the sole carbon source achieving a maximum decolorization of 60% after 9 days of incubation and producing a red-colored metabolite. In contrast, Bacillus BT5 and Lysinibacillus BT32 exhibited the lowest percentages of decolorization, barely 33% after 16 and 11 days of incubation, respectively. When Aspergillus H1T was grown in indigo dye supplemented with glucose, 96% of decolorization was reached after 2 days. This study demonstrates the valorization of denim textile effluents for the production of hydrogen via dark fermentation with concomitant color removal.

中文翻译：

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牛仔纺织废水的同步制氢和脱色：细菌和真菌菌株对靛蓝染料脱色的动力学

本研究提出了通过发酵制氢过程处理和回收牛仔纺织废水。此外，该研究还展示了从发酵纺织废水中获得的细菌和真菌分离物的脱色能力。最大产氢率为0.23 LH 2 /Ld，同时实现脱色。从发酵的流出物中总共获得了 35 种细菌和一种真菌分离物，并筛选了它们使靛蓝染料脱色的能力，用作模型分子。从它们中，鉴定为芽孢杆菌 BT5、芽孢杆菌 BT9、乳杆菌 BT20、赖氨酸杆菌 BT32 和曲霉 H1T 的分离株显示出显着的脱色能力。乳酸菌 BT20 使用葡萄糖作为共底物在培养 11 天后达到 90% 的脱色，产生无色代谢物。芽孢杆菌 BT9 能够利用靛蓝染料作为唯一的碳源，在培养 9 天后实现 60% 的最大脱色并产生红色代谢物。相比之下，芽孢杆菌 BT5 和赖氨酸芽孢杆菌 BT32 的脱色百分比最低，在孵育 16 天和 11 天后分别仅为 33%。当 Aspergillus H1T 在补充有葡萄糖的靛蓝染料中生长时，2 天后达到 96% 的脱色。该研究证明了通过伴随颜色去除的暗发酵来生产氢气的牛仔布纺织品流出物的价值化。芽孢杆菌 BT5 和赖氨酸芽孢杆菌 BT32 的脱色百分比最低，在孵育 16 天和 11 天后分别仅为 33%。当 Aspergillus H1T 在补充有葡萄糖的靛蓝染料中生长时，2 天后达到 96% 的脱色。该研究证明了通过伴随颜色去除的暗发酵来生产氢气的牛仔布纺织品流出物的增值。芽孢杆菌 BT5 和赖氨酸芽孢杆菌 BT32 的脱色百分比最低，在孵育 16 天和 11 天后分别仅为 33%。当 Aspergillus H1T 在补充有葡萄糖的靛蓝染料中生长时，2 天后达到 96% 的脱色。该研究证明了通过伴随颜色去除的暗发酵来生产氢气的牛仔布纺织品流出物的增值。