Stable recombinant mammalian cells are of growing importance in pharmaceutical biotechnology production scenarios for biologics such as monoclonal antibodies, growth and blood factors, cytokines and subunit vaccines. However, the establishment of recombinant producer cells using classical stable transfection of plasmid DNA is hampered by low stable gene transfer efficiencies. Consequently, subsequent selection of transgenic cells and the screening of clonal cell populations are time- and thus cost-intensive. To overcome these limitations, expression cassettes were embedded into transposon-derived donor vectors. Upon the co-transfection with transposase-encoding constructs, elevated vector copy numbers stably integrated into the genomes of the host cells are readily achieved facilitating under stringent selection pressure the establishment of cell pools characterized by sustained and high-yield recombinant protein production. Here, we discuss some aspects of transposon vector technologies, which render these vectors promising candidates for their further utilization in the production of biologics.

中文翻译：

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转座子载体介导的稳定基因转移，用于加速建立重组哺乳动物细胞库，从而实现生物制剂的高产生产

稳定的重组哺乳动物细胞在生物制剂的制药生物技术生产场景中越来越重要，例如单克隆抗体、生长和血液因子、细胞因子和亚单位疫苗。然而，使用经典的质粒 DNA 稳定转染建立重组生产细胞受到稳定基因转移效率低的阻碍。因此，随后转基因细胞的选择和克隆细胞群的筛选是时间密集型和成本密集型的​​。为了克服这些限制，将表达盒嵌入转座子衍生的供体载体中。在与转座酶编码构建体共转染后，稳定整合到宿主细胞基因组中的高载体拷贝数很容易实现，这有助于在严格的选择压力下建立以持续和高产重组蛋白生产为特征的细胞库。在这里，我们讨论转座子载体技术的一些方面，这些技术使这些载体成为进一步用于生物制剂生产的候选者。