The GH (growth hormone)/IGFs (insulin-like growth factors) system has an important function in the regulation of growth. In this system, IGFBPs play a crucial regulatory role in IGF functions. As a member of the IGFBP family, IGFBP2 can bind to IGF and regulate IGF functions to regulate development and growth. In addition, IGFBP2 shows key regulatory functions in cell proliferation and metabolism. In this study, the igfbp2 gene was cloned from grass carp (Ctenopharyngodon idellus) liver. The ORF of grass carp igfbp2 is 834 bp long and encodes 277 amino acids. The tissue distribution results showed that igfbp2 is expressed in multiple tissues in grass carp and has a high expression level in the liver. In the OGTT, igfbp2 expression was significantly decreased in the liver and brain after 6 h of treatment with glucose. In vitro, igfbp2 expression in grass carp's primary hepatocytes was significantly suppressed by insulin after treatment for 6 and 12 h. Moreover, igfbp2 expression was markedly increased in a dose-dependent manner with glucagon incubation in grass carp's primary hepatocytes. To the best of our knowledge, this is the first report about Igfbp2 in grass carp. These results will provide a basis for the in-depth study of grass carp Igfbp2.

中文翻译：

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草鱼igfbp2的分子鉴定以及葡萄糖，胰岛素和胰高血糖素对igfbp2 mRNA表达的影响。

GH（生长激素）/ IGF（胰岛素样生长因子）系统在调节生长中具有重要作用。在这个系统中，IGFBP在IGF功能中起着至关重要的调节作用。作为IGFBP家族的成员，IGFBP2可以与IGF结合并调节IGF的功能来调节发育和生长。此外，IGFBP2在细胞增殖和代谢中显示出关键的调节功能。在这项研究中，igfbp2基因是从草鱼（Ctenopharyngodon idellus）肝脏克隆的。草鱼igfbp2的ORF长834 bp，编码277个氨基酸。组织分布结果表明，igfbp2在草鱼的多种组织中表达，在肝脏中具有较高的表达水平。在OGTT中，葡萄糖治疗6小时后，igfbp2表达在肝脏和大脑中显着降低。体外，处理6h和12h后，胰岛素显着抑制了草鱼初级肝细胞igfbp2的表达。此外，随着胰高血糖素在草鱼原代肝细胞中的孵育，igfbp2表达以剂量依赖性方式显着增加。据我们所知，这是草鱼中有关Igfbp2的第一个报道。这些结果将为深入研究草鱼Igfbp2奠定基础。