Trehalose is a non-reducing disaccharide composed of two α-glucose molecules and synthesized by an enzyme complex containing four subunits TPS1 (EC 2.4.1.15), TPS2 (EC 3.1.3.12), TPS3, and TSL1. First reports about trehalose classified this sugar as an energy reserve compound like glycogen. However, lately, trehalose is known to assist yeast cells during heat, osmotic, and starvation stresses. In Saccharomyces cerevisiae, the deletion of the tps1 encoding gene eliminated the yeast ability to grow on glucose as the sole carbon source. Kluyveromyces lactis is a yeast present in various dairy products and is currently utilized for the synthesis of more than 40 industrial heterologous products. In this study, the deletion of the tps1 gene in K. lactis showed that unlike S. cerevisiae, tps1 gene disruption does not cause growth failure in glucose, galactose, or fructose. The µMAX rate values of K. lactis tps1Δ strains were equal than the non-disrupted strains, showing that the gene deletion does not affect the yeast growth. After gene disruption, the absence of trehalose into the metabolism of K. lactis was also confirmed.

中文翻译：

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乳酸克鲁维酵母中海藻糖tps1基因的删除不会损害葡萄糖的生长。

海藻糖是由两个α-葡萄糖分子组成的非还原性二糖，由包含四个亚基TPS1，EC2.4.1.15，TPS2（EC3.1.3.12），TPS3和TSL1的酶复合物合成。关于海藻糖的第一份报道将这种糖归类为糖原等能量储备化合物。然而，近来，已知海藻糖在高温，渗透压和饥饿胁迫下辅助酵母细胞。在酿酒酵母中，tps1编码基因的缺失消除了酵母以葡萄糖作为唯一碳源生长的能力。乳酸克鲁维酵母是存在于各种乳制品中的酵母，目前被用于合成40多种工业异源产品。在这项研究中，乳酸克鲁维酵母中tps1基因的缺失表明，与酿酒酵母不同，tps1基因破坏不会导致葡萄糖的生长衰竭，半乳糖或果糖。乳酸克鲁维酵母tps1Δ菌株的μMAX速率值与未破坏的菌株相同，这表明基因缺失不影响酵母的生长。基因破坏后，还证实了海藻糖不参与乳酸克鲁维酵母的代谢。