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Overexpression of LINC00691 promotes the proliferation and invasion of gastric cancer cells via the Janus kinase/signal transducer and activator of transcription signalling pathway.
The International Journal of Biochemistry & Cell Biology ( IF 4 ) Pub Date : 2020-04-21 , DOI: 10.1016/j.biocel.2020.105751
Wei Liang 1 , Bin Xia 2 , Chao He 3 , Guanghua Zhai 1 , Meifen Li 1 , Jundong Zhou 3
Affiliation  

This report aims to explore how LINC00691 regulates the proliferation and invasion of gastric cancer (GC). Clinical tissue and serum samples, as well as specimens in the Cancer Genome Atlas (TCGA) database, were used to analyse the expression of LINC00691 in GC. Our data indicated that the expression of LINC00691 in GC was significantly higher than that in healthy controls and was associated with clinicopathological features and survival time. In the GC cell lines MKN-45 and HGC-27, the knockdown of LINC00691 suppressed proliferation, colony formation, migration, and invasion. Bioinformatics analysis and luciferase reporter gene experiments showed that LINC00691 activated Lin28 transcription. Western blot analysis indicated that the knockdown of LINC00691 contributed to the decreased expression of p-JAK2 and p-STAT3 in GC cells. The Janus kinase/signal transducer and activator of transcription (JAK/STAT) signalling pathway inhibitor ruxolitinib effectively suppressed the effects of LINC00691. In addition, both LINC00691 and Lin28 promoted the expression of epidermal growth factor (EGF). Therefore, our study clarified that LINC00691 is highly expressed in GC and is a potential biomarker for GC diagnosis and prognosis. LINC00691 promotes the proliferation and invasion of GC cells by activating Lin28 transcription and facilitating EGF expression through the JAK/STAT signalling pathway, which provides new ideas for targeted therapy of GC.



中文翻译:

LINC00691的过表达通过Janus激酶/信号转导和转录信号通路激活剂促进胃癌细胞的增殖和侵袭。

本报告旨在探讨LINC00691如何调节胃癌 (GC) 的增殖和侵袭。临床组织和血清样本以及癌症基因组图谱 (TCGA) 数据库中的标本用于分析LINC00691在 GC 中的表达。我们的数据表明,LINC00691在 GC中的表达显着高于健康对照,并且与临床病理特征和存活时间相关。在 GC 细胞系 MKN-45 和 HGC-27 中,LINC00691的敲低抑制了增殖、集落形成、迁移和侵袭。生物信息学分析和荧光素酶报告基因实验表明LINC00691激活了Lin28转录。蛋白质印迹分析表明,LINC00691的敲低导致 GC 细胞中 p-JAK2 和 p-STAT3 的表达降低。Janus 激酶/信号转导和转录激活剂 (JAK/STAT) 信号通路抑制剂鲁索替尼有效抑制了LINC00691的作用。此外,LINC00691Lin28均促进表皮生长因子(EGF)的表达。因此,我们的研究阐明了LINC00691在 GC 中高表达,是 GC 诊断和预后的潜在生物标志物。LINC00691通过激活Lin28促进GC细胞增殖和侵袭 通过 JAK/STAT 信号通路转录并促进 EGF 表达,为 GC 的靶向治疗提供了新思路。

更新日期:2020-04-21
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