Abstract The development of an accurate and low-cost monitoring technique for hydrogen peroxide (H2O2) is a crucial demand in environment, food industry, medicine and biology. Herein, we report the design and synthesis of viologen terminated second (G2.0) and third generation (G3.0) poly(amidoamine) PAMAM dendrimers, followed by encapsulation with gold nanoparticles to form G2.0 and G3.0 Vio-PAMAM-AuNPs. The G2.0 and G3.0 Vio-PAMAM-AuNPs were deposited over glassy carbon electrode (GCE) to form G2.0 and G3.0 Vio-PAMAM-AuNPs/GCE modified electrodes, respectively. The electrochemical behavior of G2.0 and G3.0 Vio-PAMAM-AuNPs/GCEs were investigated using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). Both the G2.0 and G3.0 Vio-PAMAM-AuNPs/GCEs showed a pair of well-defined redox peaks in 0.1 M phosphate buffer corresponding to the redox behavior of viologen V2+↔V•+ radical. G3.0 Vio-PAMAM-AuNPs/GCE has shown a higher current response than that of the G2.0 Vio-PAMAM-AuNPs/GCE and further the G3.0 Vio-PAMAM-AuNPs/GCE demonstrated impressive electrocatalytic activity towards reduction of H2O2, based on which a nonenzymatic sensor for the detection of H2O2 has been developed. The developed nonenzymatic sensor has displayed excellent performance towards H2O2 detection in the broad linear range of 0.1 mM – 6.2 mM with a low detection limit of 27 μM and high sensitivity of 202.7 μA mM−1 cm−2. The G3.0 Vio-PAMAM-AuNPs/GCE modified electrode with its extensive dendritic structure creating tailored sanctuary to accommodate a large number of viologen mediator and AuNPs exhibited good operational and long term stability and further the quantification of H2O2 in real samples has been verified by standard addition method.

中文翻译：

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紫罗碱封端的聚酰胺胺 (PAMAM) 树枝状大分子包裹有金纳米粒子，用于非酶法测定过氧化氢

摘要 开发一种准确、低成本的过氧化氢 (H2O2) 监测技术是环境、食品工业、医学和生物学领域的关键需求。在此，我们报告了紫精终止的第二代 (G2.0) 和第三代 (G3.0) 聚（酰氨基胺）PAMAM 树枝状聚合物的设计和合成，然后用金纳米颗粒封装以形成 G2.0 和 G3.0 Vio-PAMAM -AuNP。G2.0 和 G3.0 Vio-PAMAM-AuNPs 沉积在玻璃碳电极 (GCE) 上，分别形成 G2.0 和 G3.0 Vio-PAMAM-AuNPs/GCE 修饰电极。使用电化学阻抗谱 (EIS) 和循环伏安法 (CV) 研究了 G2.0 和 G3.0 Vio-PAMAM-AuNPs/GCEs 的电化学行为。G2.0 和 G3.0 Vio-PAMAM-AuNPs/GCEs 在 0 中都显示出一对明确的氧化还原峰。1 M 磷酸盐缓冲液，对应于紫精 V2+↔V•+ 自由基的氧化还原行为。G3.0 Vio-PAMAM-AuNPs/GCE 显示出比 G2.0 Vio-PAMAM-AuNPs/GCE 更高的电流响应，而且 G3.0 Vio-PAMAM-AuNPs/GCE 显示出令人印象深刻的电催化活性，可减少H2O2，在此基础上开发了一种用于检测 H2O2 的非酶传感器。开发的非酶传感器在 0.1 mM – 6.2 mM 的宽线性范围内对 H2O2 检测显示出优异的性能，检测限为 27 μM，灵敏度为 202.7 μA mM-1 cm-2。G3。