当前位置: X-MOL 学术J. Neurosci. Methods › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
High-resolution MEMRI characterizes laminar specific ascending and descending spinal cord pathways in rats.
Journal of Neuroscience Methods ( IF 3 ) Pub Date : 2020-04-23 , DOI: 10.1016/j.jneumeth.2020.108748
Vijai Krishnan 1 , Jiadi Xu 2 , Albert German Mendoza 2 , Alan Koretsky 3 , Stasia A Anderson 4 , Galit Pelled 5
Affiliation  

BACKGROUND The spinal cord is composed of nine distinct cellular laminae that currently can only be visualized by histological methods. Developing imaging methods that can visualize laminar architecture in-vivo is of significant interest. Manganese enhanced magnetic resonance imaging (MEMRI) yields valuable architectural and functional information about the brain and has great potential in characterizing neural pathways in the spinal cord. Here we apply MEMRI to visualize laminae architecture in the thoracic region of the spinal cord with ultra-high resolution. NEW METHOD Manganese chloride (MnCl2) was delivered systemically and imaging of the lumbar and thoracic spinal cord levels was acquired in high field, 11.7 T MRI scanner, 48 h following MnCl2 administration. RESULTS Here we demonstrate laminar specific signal enhancement in the spinal cord of rats administered with MnCl2 with 69 μm in-plane resolution. We also report reduced T1 values over time in MnCl2 groups across laminae IIX. COMPARISONS WITH EXISTING METHODS This is the first study to demonstrate that MEMRI is capable of identifying spinal laminae at a high resolution of 69 μm in a living animal. This would enable the visualization of architecture and function of distinct regions with improved resolution, in healthy and diseased animal models. CONCLUSIONS The regions with the largest T1 enhancements were observed to correspond to laminae that contain either high cell density or large motor neurons, making MEMRI an excellent tool for studying spinal cord architecture, physiology and function in different animal models.

中文翻译:

高分辨率 MEMRI 表征了大鼠层状特定的脊髓上升和下降通路。

背景脊髓由九个不同的细胞层组成,目前只能通过组织学方法可视化。开发能够在体内可视化层状结构的成像方法具有重要意义。锰增强磁共振成像(MEMRI)可产生有关大脑的有价值的结构和功能信息,并且在表征脊髓神经通路方面具有巨大潜力。在这里,我们应用 MEMRI 以超高分辨率可视化脊髓胸部区域的椎板结构。新方法 全身输送氯化锰 (MnCl2),并在 MnCl2 给药 48 小时后在高场 11.7 T MRI 扫描仪中获取腰椎和胸椎脊髓水平的成像。结果在这里,我们证明了给予 MnCl2 的大鼠脊髓中层状特异性信号增强,平面内分辨率为 69 μm。我们还报告了跨层 IIX 的 MnCl2 组中 T1 值随着时间的推移而降低。与现有方法的比较 这是第一项证明 MEMRI 能够以 69 μm 高分辨率识别活体动物脊椎椎板的研究。这将使健康和患病动物模型中不同区域的结构和功能以更高的分辨率可视化。结论 观察到 T1 增强最大的区域对应于含有高细胞密度或大运动神经元的椎板,使 MEMRI 成为研究不同动物模型中脊髓结构、生理学和功能的绝佳工具。
更新日期:2020-04-23
down
wechat
bug