Abstract Microorganisms from oil reservoirs are associated with negative impacts on the oilfield, such as oil biodeterioration and biocorrosion of steel structures used for exploration, transportation and storage. The objectives of this study were to analyze the taxonomic and functional diversity in the production water and oil of two oil reservoirs, with emphasis on H2S producing bacteria. Total DNA from the microbial community was extracted, and the taxonomic and functional diversity was evaluated. The phyla Proteobacteria, Firmicutes and Euryarchaeota were the most abundant among the samples and contain the main sulfate-reducing species, such as Desulfovibrio alaskensis and Archeoglobus fulgidus. The production water and the oil were found to have taxonomic and functionally different microbial communities in the three communities, however correlation networks showed basically the same types of interconnected metabolism between the sulfidogenic microorganisms. This indicates the importance of understanding the microbial diversity and metabolic capacity of communities in these environments and trying to control harmful processes that may result in huge economic damage to the oil industry.

中文翻译：

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石油生产水和油样中硫酸盐还原原核生物的多样性

摘要 油藏微生物对油田产生不利影响，如石油生物变质和用于勘探、运输和储存的钢结构的生物腐蚀。本研究的目的是分析两个油藏生产水和油的分类和功能多样性，重点是产生 H2S 的细菌。提取微生物群落的总 DNA，并评估分类和功能多样性。Proteobacteria、Firmicutes 和 Euryarchaeota 门是样品中最丰富的，包含主要的硫酸盐还原物种，如 Desulfovibrio alaskensis 和 Archeoglobus fulgidus。发现生产水和油在三个群落中具有分类和功能不同的微生物群落，然而，相关网络显示硫化微生物之间的相互关联的代谢类型基本相同。这表明了解这些环境中群落的微生物多样性和代谢能力并试图控制可能对石油工业造成巨大经济损失的有害过程非常重要。