Artemisia annua is well known for biosynthesizing the antimalarial drug artemisinin. Here, a global proteomic profiling of A. annua is conducted with identification of a total of 13 403 proteins based on the genome sequence annotation database. Furthermore, a spectral library is generated to perform quantitative proteomic analysis using data independent acquisition mass spectrometry. Specifically, proteins between two chemotypes that produce high (HAP) and low (LAP) artemisinin content, respectively, are comprehensively quantified and compared. 182 proteins are identified with abundance significantly different between these two chemotypes means after the statistic use the p-value and fold change it is found 182 proteins can reach the demand conditions which represent the expression are significantly different between the high artemisnin content plants (HAPs) and the low artemisnin content plants (LAPs). Data are available via ProteomeXchange with identifier PXD015547. Overall, this current study globally identifies the proteome of A. annua and quantitatively compares the targeted sub-proteomes between the two cultivars of HAP and LAP, providing systematic information on metabolic pathways of A. annua.

中文翻译：

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青蒿蛋白质组的综合图谱和产生高与低青蒿素含量的化学型中差异蛋白质表达的定量。

青蒿以生物合成抗疟药青蒿素而闻名。在这里，对青蒿进行了全球蛋白质组学分析，根据基因组序列注释数据库共鉴定了 13 403 种蛋白质。此外，生成光谱库以使用数据独立采集质谱法进行定量蛋白质组学分析。具体而言，对分别产生高 (HAP) 和低 (LAP) 青蒿素含量的两种化学型之间的蛋白质进行了全面量化和比较。182种蛋白质被鉴定，这两种化学型之间的丰度显着差异意味着在统计使用p值和倍数变化后发现182种蛋白质可以达到代表高青蒿素含量植物（HAPs）之间表达显着差异的需求条件以及低青蒿素含量植物 (LAP)。数据可通过 ProteomeXchange 获得，标识符为 PXD015547。总体而言，目前的这项研究在全球范围内确定了青蒿的蛋白质组，并定量比较了 HAP 和 LAP 两个品种之间的靶向亚蛋白质组，提供了关于青蒿代谢途径的系统信息。