Haprin (TRIM36) is a ubiquitin‐protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. It is expressed in the testes in both mice and humans and is thought to be involved in spermiogenesis, the acrosome reaction, and fertilization. However, the functional role of Haprin is poorly understood. The aim of this study was to investigate the physiological role of Haprin in fertility. Homozygous haprin ‐deficient mice were generated and these mice, and their spermatozoa, were analyzed to detect morphological and fertility‐related abnormalities. In these models, normal spermatogenesis was observed but sperm quality was reduced with haprin ‐deficient mice having poorer sperm morphology and motility than wild‐type mice. Interestingly, haprin ‐deficient mice showed normal in vivo fertility but could not fertilize oocytes under standard in vitro fertilization conditions. In conclusion, this study demonstrated that Haprin deficiency causes morphological abnormalities in spermatozoa, indicating that Haprin is involved in spermiogenesis.

中文翻译：

---

缺乏 Haprin 的精子不能进行体外受精。

Haprin (TRIM36) 是一种泛素蛋白连接酶，可介导靶蛋白的泛素化和随后的蛋白酶体降解。它在小鼠和人类的睾丸中都有表达，被认为与精子发生、顶体反应和受精有关。然而，人们对 Haprin 的功能作用知之甚少。本研究的目的是调查 Haprin 在生育中的生理作用。产生了纯合haprin 缺陷小鼠，并对这些小鼠及其精子进行分析，以检测形态学和生育相关异常。在这些模型中，观察到正常的精子发生，但与野生型小鼠相比， haprin 缺陷小鼠的精子形态和运动能力较差，精子质量降低。有趣的是，haprin 缺陷小鼠体内生育力正常，但不能在标准体外受精条件下使卵母细胞受精。总之，本研究表明，Haprin 缺乏会导致精子形态异常，表明 Haprin 参与了精子发生。