Monilinia fructicola is the most widely distributed species among the Monilinia genus in the world, and causes blossom blight, twig canker, and fruit rot on Rosaceae fruits. To date, studies on genomics and pathogenicity are limited in M. fructicola. In this study, we identified a redox‐related gene, MfOfd1, which was significantly up‐regulated at 1 hr after inoculation of M. fructicola on peach fruits. We used the clustered regulatory inter‐spaced short palindromic repeats (CRISPR)/Cas9 system combined with homologous recombination to determine the function of the MfOfd1 gene. The results showed that the sporulation of knockdown transformants was reduced by 53% to 83%. The knockdown transformants showed increased sensitivity to H₂O₂ and decreased virulence on peach fruits compared to the wild‐type isolate Bmpc7. It was found that H₂O₂ could stimulate the expression of MfOfd1 in the wild‐type isolate. The transformants were also more sensitive to exogenous osmotic stress, such as glycerol, d‐sorbitol, and NaCl, and to dicarboximide fungicides (iprodione and dimethachlon). These results indicate that the MfOfd1 gene plays an important role in M. fructicola in sporulation, oxidative response, osmotic stress tolerance, and virulence.

中文翻译：

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MfOfd1对于桃褐腐真菌莫妮莉亚fructicola中的胁迫响应和毒力至关重要。

莫尼利尼亚果蝇是世界上莫尼利尼亚属中分布最广的物种，会引起蔷薇科水果开花枯萎，树枝枯萎和果实腐烂。迄今为止，在果蝇中对基因组学和致病性的研究是有限的。在这项研究中，我们发现了氧化还原相关基因，MfOfd1，这是显著在1个小时的接种后上调M.腐病菌对桃果实。我们使用簇状调控间隔回文短回文重复序列（CRISPR）/ Cas9系统与同源重组相结合来确定MfOfd1的功能基因。结果表明，敲低的转化体的孢子形成减少了53％至83％。与野生型分离株Bmpc7相比，敲低的转化株显示出对H ₂ O _2的敏感性提高，桃果实的毒力降低。发现H ₂ O ₂可以刺激野生型分离物中MfOfd1的表达。转化株对甘油，d-山梨糖醇和NaCl等外源渗透胁迫以及双羧酰亚胺杀真菌剂（异丙洛酮和二甲双胍）也更敏感。这些结果表明，MfOfd1基因在M. fructicola中起重要作用。 在孢子形成，氧化反应，渗透胁迫耐受性和毒力方面。