microRNA‐3607 (miR‐3607) has been identified as an important biomarker, and its aberrant expression exerts a significant role in tumorigenesis. However, the biological function of miR‐3607 in hepatocellular carcinoma (HCC) needs to be deciphered comprehensively. Clinical samples of HCC patients, as well as normal cases, were derived from The Cancer Genome Atlas database. Quantitative reverse transcription polymerase chain reaction (qRT‐PCR) and Western blotting analyses were utilized to detect the expression levels of indicated genes. Cell counting kit‐8 (CCK‐8), colony formation, and transwell assays were performed to assess the effect of miR‐3607 in HCC cell viability, migration, and invasion. Bioinformatics analysis and luciferase reporter gene assay was applied to screen the target genes of miR‐3607 and verified the association between miR‐3607 and its potential target gene. Our study showed that miR‐3607 expression was decreased in HCC tissues and cell lines, and its downregulation was linked with poor outcomes of HCC patients. miR‐3607 was noted to inhibit HCC cell growth, colony formation, migration, and invasion. Besides, minichromosome maintenance (MCM5) was a possible target gene of miR‐3607 in HCC. Overexpression of MCM5 was observed in HCC and induced unfavorable prognosis. MCM5 expression had a negative correlation with miR‐3607. MCM5 can abolish the suppressive impacts of miR‐3607 on HCC cell malignant behaviors and the epithelial‐mesenchymal transition (EMT) process. To sum up, our results unveiled that miR‐3607 could inhibit HCC cell growth, migration, and invasion by regulating MCM5 and mediating EMT process, suggesting a new probable biomarker for further treatment of HCC.

中文翻译：

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miR-3607，肝细胞癌侵袭和侵袭性的生物标志物：其与上皮间质转化过程的关系

microRNA-3607 (miR-3607) 已被确定为重要的生物标志物，其异常表达在肿瘤发生中发挥重要作用。然而，miR-3607在肝细胞癌（HCC）中的生物学功能需要全面破译。HCC 患者以及正常病例的临床样本来自癌症基因组图谱数据库。定量逆转录聚合酶链反应 (qRT-PCR) 和蛋白质印迹分析用于检测指定基因的表达水平。进行细胞计数试剂盒-8 (CCK-8)、集落形成和 transwell 测定以评估 miR-3607 对 HCC 细胞活力、迁移和侵袭的影响。应用生物信息学分析和荧光素酶报告基因检测筛选 miR-3607 的靶基因，验证 miR-3607 与其潜在靶基因之间的关联。我们的研究表明 miR-3607 在 HCC 组织和细胞系中的表达降低，其下调与 HCC 患者的不良预后有关。miR-3607 可抑制 HCC 细胞生长、集落形成、迁移和侵袭。此外，微染色体维持（MCM5）可能是 miR-3607 在 HCC 中的靶基因。在 HCC 中观察到 MCM5 的过度表达并导致不利的预后。MCM5 表达与 miR-3607 呈负相关。MCM5 可以消除 miR-3607 对 HCC 细胞恶性行为和上皮间质转化 (EMT) 过程的抑制作用。总结，