In this article, for the first time, the electrochemical properties of a novel pyridine derivative, 4‐(2‐(2‐hydroxybenzylidene) hydrazinyl)‐1‐(3‐phenylpropyl) pyridinium bromide (abbreviated as 4‐Pyri), and its interaction with double stranded DNA (dsDNA) was investigated. The interaction between candidate drug molecule (4‐Pyri) and dsDNA was analyzed by examining 4‐Pyri (+0.6 V and +0.8 V) and guanine (+1.0 V) oxidation signal changes with Differential Pulse Voltammetry (DPV) and Cyclic Voltammetry (CV). Electrochemical Impedance Spectroscopy (EIS) was used to show the resistance changes before and after the interaction between 4‐Pyri and dsDNA. We showed that after the interaction with 4‐Pyri, the oxidation currents of guanine decreased dramatically, whereas the intrinsic oxidation currents of 4‐Pyri dramatically increased. 4‐Pyri oxidation current differences before and after the interaction with dsDNA enabled us to determine such interaction separately from guanine oxidation signals. In addition, resistance differences were observed at before and after the interaction with each other that confirmed the possible interaction. In addition, toxicity effect (S%) value, which is an important parameter for electrochemical studies indicated 4‐Pyri's toxicity to dsDNA. Our results demonstrated that 4‐Pyri interacts with dsDNA, and could be used as a potential candidate drug molecule due to its remarkable impact on dsDNA.

中文翻译：

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新型吡啶盐作为候选药物分子的电化学特性及其与DNA的相互作用

本文首次提出了一种新型吡啶衍生物4-（2-（2-羟基苄叉）肼基）-1-（3-苯丙基）溴化吡啶鎓（缩写为4-Pyri）的电化学性质，并研究了其与双链DNA（dsDNA）的相互作用。通过用差动脉冲伏安法（DPV）和循环伏安法（4-Vyri）检测4-Pyri（+0.6 V和+0.8 V）和鸟嘌呤（+1.0 V）氧化信号变化来分析候选药物分子（4-Pyri）与dsDNA之间的相互作用简历）。电化学阻抗谱（EIS）用于显示4-Pyri与dsDNA相互作用之前和之后的电阻变化。我们发现与4-Pyri相互作用后，鸟嘌呤的氧化电流急剧下降，而4-Pyri的固有氧化电流急剧增加。与dsDNA相互作用前后的4-Pyri氧化电流差异使我们能够与鸟嘌呤氧化信号分开确定这种相互作用。此外，在彼此相互作用之前和之后观察到电阻差异，证实了可能的相互作用。此外，作为电化学研究的重要参数的毒性效应（S％）值表明4-Pyri对dsDNA的毒性。我们的结果表明4-Pyri与dsDNA相互作用，由于其对dsDNA的显着影响，因此可以用作潜在的候选药物分子。