Our heart is comprised of many different cell types that all contribute to cardiac function. An important step in deciphering the molecular complexity of our heart is to decipher the molecular composition of the various cardiac cell types. Here we set out to delineate a comprehensive protein expression profile of the two most prevalent cell types in the heart: cardiomyocytes and cardiac fibroblasts. To this end, we isolated cardiomyocytes and fibroblasts from rat hearts and combined state-of-the-art flow cytometry with high-resolution mass spectrometry to investigate their proteome profiles right after isolation. We measured and quantified 5240 proteins in cardiomyocytes and 6328 proteins in cardiac fibroblasts. In addition to providing a global protein profile for these cardiac cell types, we also present specific findings, such as unique expression of ion channels and transcription factors for each cell type. For instance, we show that the sodium channel Scn7a and the cation channel Trpm7 are expressed in fibroblasts but not in cardiomyocytes, which underscores the importance of investigating the endogenous cell host prior to functional studies. Our dataset represents a valuable resource on protein expression profiles in these two primary cardiac cells types.

中文翻译：

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急性分离的成年原代大鼠心肌细胞和成纤维细胞的定量蛋白质组学表征。

我们的心脏由许多不同的细胞类型组成，所有这些类型的细胞都有助于心脏功能。解读我们心脏的分子复杂性的一个重要步骤是解读各种心脏细胞类型的分子组成。在这里，我们着手描绘心脏中两种最普遍的细胞类型：心肌细胞和心脏成纤维细胞的全面蛋白质表达谱。为此，我们从大鼠心脏中分离了心肌细胞和成纤维细胞，并结合了最新的流式细胞术和高分辨率质谱技术，以在分离后立即研究其蛋白质组谱。我们测量并定量了心肌细胞中的5240种蛋白质和心脏成纤维细胞中的6328种蛋白质。除了提供这些心肌细胞类型的全局蛋白质谱外，我们还提出了一些具体的发现，例如每种细胞类型的离子通道和转录因子的独特表达。例如，我们显示钠通道Scn7a和阳离子通道Trpm7在成纤维细胞中表达，但在心肌细胞中不表达，这强调了在功能研究之前研究内源性细胞宿主的重要性。我们的数据集代表了这两种主要心脏细胞类型中蛋白质表达谱的宝贵资源。