Adipose-derived stem cells (ASCs) have neuroprotective effects, and their repair ability has been approved in neurodegenerative studies. Pregnenolone as a neurosteroid plays significant roles in neurogenesis. We aimed to consider the effect of ADSCs and pregnenolone injection on the multiple sclerosis (MS) model created by cuprizone. Male Wistar rats (n = 36) were fed with an ordinary diet or a diet with cuprizone (0.6%) for 3 weeks. H-ADSCs were taken from patients with lipoaspirate surgery. The rats were divided into six groups (n = 6): healthy, MS, sham, pregnenolone injection, ADSCs injection, pregnenolone and ADSCs injection. Behavioral test, histological examination and TEM were conducted. The specific markers for myelin and cell differentiation were assessed using immunohistochemistry staining. Additionally, the measure of MBP and MOG gene expression and the amount of related proteins were determined using real-time RT-PCR and ELISA techniques, respectively. Histologic results showed that induced demyelination in corpus callosum fibers. TEM revealed an increased thickness of myelin in fibers in the treated groups (P < 0.05). Injection of hADSC and pregnenolone significantly increased the expression levels of MBP and MOG (P < 0.001). The mean percentage of MOG and MBP markers were significantly increased in the treated groups compared to MS and sham groups (P < 0.05). Moreover, the OD level of MBP and MOG proteins showed that their values in the ADSCs/pregnenolone group were close to those of the control group without a significant difference. Our data indicated the remyelination potency and cell differentiation can improve with ADSCs and pregnenolone treatments in the multiple sclerosis model which created by cuprizone in rats.

中文翻译：

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在多发性硬化症的cuprizone大鼠模型中，使用人类脂肪干细胞（hADSC）和孕烯醇酮改善脱髓质体中的髓鞘再生。

脂肪干细胞（ASC）具有神经保护作用，其修复能力已在神经退行性研究中得到认可。孕烯醇酮作为神经甾体在神经发生中起重要作用。我们旨在考虑ADSCs和孕烯醇酮注射液对cuprizone建立的多发性硬化（MS）模型的影响。雄性Wistar大鼠（n  = 36）接受普通饮食或含铜酮（0.6％）的饮食3周。H-ADSCs来自抽脂手术患者。将大鼠分为六组（n  = 6）：健康，MS，假，孕烯醇酮注射液，ADSCs注射液，孕烯醇酮和ADSCs注射液。进行行为测试，组织学检查和TEM。使用免疫组织化学染色评估髓鞘和细胞分化的特异性标记。此外，分别使用实时RT-PCR和ELISA技术确定MBP和MOG基因表达的量度以及相关蛋白的量。组织学结果表明，induced体纤维中引起脱髓鞘。TEM显示，治疗组的纤维中髓磷脂厚度增加（P  <0.05）。注射hADSC和孕烯醇酮显着增加MBP和MOG的表达水平（P <0.001）。与MS和假手术组相比，治疗组的MOG和MBP标记物的平均百分比显着增加（P <0.05）。此外，MBP和MOG蛋白的OD值表明，在ADSCs /孕烯醇酮组中它们的值接近于对照组，而没有显着差异。我们的数据表明，在cuprizone在大鼠中建立的多发性硬化模型中，ADSCs和孕烯醇酮治疗可以改善髓鞘再生能力和细胞分化。