Enterovirus A71 (EV71), the main etiological agent of handfoot- mouth disease (HFMD), circulates in many areas of the world and has caused large epidemics since 1997, especially in the Asia-Pacific region. In this study, we determined the full-genome sequence of CMC718, a newly isolated EV71 strain in Korea. The CMC718 genome was 7,415 nucleotides in length and was confirmed by whole-genome phylogenetic analysis to belong to the B5 genotype. In particular, CMC718 demonstrated maximum identity with strain M988 of the B5 genotype and numerous amino acid variants were detected in the 3D domain of the viral protein P3, which is consistent with the mutation pattern of a B5 strain isolated in 2012–2013. Comparison of the CMC718 sequence with other EV71 reference strains confirmed the relationship and genetic variation of CMC718. Our study was a full-genome sequence analysis of the first EV71 strain of the B5 genotype isolated in South Korea. This information will be a valuable reference for the development of methods for the detection of recombinant viruses, the tracking of infections, and the diagnosis of EV71.

中文翻译：

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在韩国分离的肠道病毒71的第一个B5亚基因组菌株的序列分析。

肠病毒A71（EV71）是手足口病（HFMD）的主要病原体，在世界许多地区流行，自1997年以来就引起了大规模的流行，特别是在亚太地区。在这项研究中，我们确定了韩国新分离的EV71株CMC718的全基因组序列。CMC718基因组的长度为7,415个核苷酸，并通过全基因组系统发育分析确认属于B5基因型。特别是，CMC718与B5基因型的M988菌株具有最大的同一性，并且在病毒蛋白P3的3D域中检测到许多氨基酸变体，这与2012-2013年分离的B5菌株的突变模式一致。CMC718序列与其他EV71参考菌株的比较证实了CMC718的关系和遗传变异。我们的研究是对韩国分离的首个B5基因型EV71株的全基因组序列分析。该信息将为开发检测重组病毒，追踪感染和诊断EV71的方法提供有价值的参考。