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Bisphenol A induces focal adhesions assembly and activation of FAK, Src and ERK2 via GPER in MDA-MB-231 breast cancer cells.
Toxicology in Vitro ( IF 3.2 ) Pub Date : 2020-04-20 , DOI: 10.1016/j.tiv.2020.104871
Rocio Castillo-Sanchez 1 , Javier Ramirez-Ricardo 2 , Elia Martinez-Baeza 2 , Pedro Cortes-Reynosa 2 , Fernando Candanedo-Gonzales 3 , Rocio Gomez 4 , Eduardo Perez Salazar 2
Affiliation  

Bisphenol A (BPA) is an industrial synthetic chemical used in the production of polycarbonate plastics and epoxy resins. Human exposition to BPA is primarily through eating food, and drinking liquids, because BPA can leach from polycarbonate plastic containers, beverage cans and epoxy resins. BPA induces proliferation and migration in human breast cancer cells. The G protein-coupled estrogen receptor (GPER) is a G protein-coupled receptor coupled with Gs proteins that is activated by estrogen and estrogenic compounds and it is the receptor for BPA. However, the signal transduction pathways that mediate migration via BPA/GPER in triple negative breast cancer (TNBC) cells has not been studied in detail. Here, we demonstrate that BPA induces an increase of GPER expression and activation of FAK, Src and ERK2, and an increase of focal adhesion assembly via GPER in TNBC MDA-MB-231 cells. Moreover, BPA induces FAK and ERK2 activation, focal adhesion assembly and migration via epidermal growth factor receptor (EGFR) transactivation. Collectively our data showed that BPA via GPER and/or EGFR transactivation induces activation of signal transduction pathways that mediate migration in TNBC MDA-MB-231 cells.

中文翻译:

双酚A在MDA-MB-231乳腺癌细胞中通过GPER诱导粘着斑组装并激活FAK,Src和ERK2。

双酚A(BPA)是一种工业合成化学品,用于生产聚碳酸酯塑料和环氧树脂。人类接触BPA的主要途径是吃食物和喝液体,因为BPA可以从聚碳酸酯塑料容器,饮料罐和环氧树脂中浸出。BPA诱导人乳腺癌细胞增殖和迁移。G蛋白偶联雌激素受体(GPER)是与Gs蛋白偶联的G蛋白偶联受体,Gs蛋白被雌激素和雌激素化合物激活,是BPA的受体。但是,尚未详细研究介导通过BPA / GPER在三阴性乳腺癌(TNBC)细胞中迁移的信号转导途径。在这里,我们证明了BPA可以诱导GPER表达增加以及FAK,Src和ERK2的激活,TNBC MDA-MB-231细胞中通过GPER引起的粘着斑组装增加。此外,BPA通过表皮生长因子受体(EGFR)反式激活诱导FAK和ERK2激活,粘着斑组装和迁移。总体而言,我们的数据表明,通过GPER和/或EGFR反式激活的BPA诱导了信号转导通路的激活,该信号转导通路介导TNBC MDA-MB-231细胞中的迁移。
更新日期:2020-04-20
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