The current research is a pioneer in the evaluation of isopyrazam biodegradation, which has been performed utilizing soil‐isolated microbes. Biodisintegrative assays of pure fungal strains, namely Aspergillus flavus (AF), Penicillium chrysogenum (PC), Aspergillus niger (AN), Aspergillus terreus (AT), and Aspergillus fumigatus (AFu), and bacterial strains, namely Xanthomonas axonopodis (XA) and Pseudomonas syringae (PS), were utilized. Initial isopyrazam concentration (10 mg/L) was prepared with an individual microbial suspension and monitored for 35 days. Isopyrazam biotransformation was analyzed quantitatively and qualitatively by UV–visible spectrophotometery and gas chromatography–mass spectroscopy. P. syringae (R2 = 0.90) and X. axonopodis (R2 = 0.88) displayed maximal potential to metabolize the fungicide (86% and 80%, respectively) while forming intermediate metabolites, including 3‐difluoromethyl‐1‐methyl‐1H‐pyrazole‐4‐carboxylic acid ((S)‐9‐hydroxy‐9‐isopropyl‐1,2,3,4‐tetrahydro‐1,4‐methanonaphthalen‐5‐yl)‐amide, 3‐difluoromethyl‐1H‐pyrazole‐4‐carboxylic acid, and 3‐difluoromethyl‐1‐methyl‐1H‐pyrazole‐4‐amide. Isopyrazam degradation by all strains, AT, PC, AFu, AN, AF, XA, and PS, was found to be 11%, 18%, 21%, 21%, 18%, 30%, 80%, and 86%, respectively, after 35 days, elucidating the effectiveness of all the utilized strains in degrading isopyrazam at varying rates. The descending order of half‐lives (days) obtained is as follows: AT (56.8) > PC (44.7) > AFu (40.7) > AN (39.6) > AF (32.6) > XA (28.1) > PS (21) days. Current research can influence imperative and significant environment‐friendly bioremedial strategies for xenobiotic eradication from the ecological compartments.

中文翻译：

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杀菌剂异吡唑啉对外源性添加的真菌和细菌菌株的降解反应

目前的研究是使用土壤分离微生物进行异吡唑啉生物降解评估的先驱。纯真菌菌株，即黄曲霉 (AF)、产黄青霉 (PC)、黑曲霉 (AN)、土曲霉 (AT) 和烟曲霉 (AFu) 以及细菌菌株，即轴突黄单胞菌 (XA) 的生物分解测定使用丁香假单胞菌(PS)。初始异吡唑啉浓度 (10 mg/L) 是用单个微生物悬浮液制备的，并监测 35 天。通过紫外-可见分光光度法和气相色谱-质谱法对异吡唑啉生物转化进行了定量和定性分析。P. syringae (R2 = 0.90) 和 X. axonopodis (R2 = 0.88) 表现出最大的代谢杀菌剂的潜力（86% 和 80%，分别）同时形成中间代谢物，包括 3-二氟甲基-1-甲基-1H-吡唑-4-羧酸（（S）-9-羟基-9-异丙基-1,2,3,4-四氢-1,4 -methanonaphthalen-5-yl)-酰胺、3-二氟甲基-1H-吡唑-4-羧酸和3-二氟甲基-1-甲基-1H-吡唑-4-酰胺。发现所有菌株 AT、PC、AFu、AN、AF、XA 和 PS 对异吡唑啉的降解率为 11%、18%、21%、21%、18%、30%、80% 和 86%，分别在 35 天后阐明所有使用的菌株以不同速率降解异吡唑啉的有效性。获得的半衰期（天）降序如下：AT（56.8）> PC（44.7）> AFu（40.7）> AN（39.6）> AF（32.6）> XA（28.1）> PS（21）天.