In this work, porous biochar derived from sugarcane bagasse was prepared and then coated with different amounts of chitosan (C@CS) for cellulase immobilization. Cellulase was covalently immobilized on the support by using glutaraldehyde as a linker. The chemical characteristics and morphology of the samples were determined by SEM, BET, FT-IR and XPS. The properties of immobilized enzyme were evaluated by activity recovery, optimum pH value and temperature, and recyclability. The results showed that all the three kinds of immobilized cellulase did not change the optimum pH value of 4 and temperature of 60 °C, and they also exhibited good activity and reusability. Especially for C@CS25 (the feeding ratio of porous biochar to chitosan was 0.5 g: 25 mg), the support retained the morphology of porous biochar well. The corresponding immobilized cellulase kept 67 % activity of free cellulase at pH = 4 and 60 °C, and showed a glucose productivity of 90.8 % even after 10 cycles.

中文翻译：

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用于戊二醛固定化高性能纤维素酶的多孔生物炭/壳聚糖复合材料

在这项工作中，制备了源自甘蔗渣的多孔生物炭，然后用不同量的壳聚糖 (C@CS) 包被以固定纤维素酶。通过使用戊二醛作为接头将纤维素酶共价固定在支持物上。通过SEM、BET、FT-IR和XPS测定样品的化学特性和形貌。固定化酶的性质通过活性恢复、最适pH值和温度以及可回收性来评价。结果表明，3种固定化纤维素酶的最适pH值4和温度60℃均未发生变化，且均表现出良好的活性和重复使用性。特别是对于C@CS25（多孔生物炭与壳聚糖的加料比为0.5 g：25 mg），载体很好地保留了多孔生物炭的形态。