当前位置: X-MOL 学术Alcohol › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
On the interaction between BDNF and serotonin systems: The effects of long-term ethanol consumption in mice.
Alcohol ( IF 2.3 ) Pub Date : 2020-04-21 , DOI: 10.1016/j.alcohol.2020.04.002
Nina K Popova 1 , Tatiana V Ilchibaeva 1 , Egor V Antonov 1 , Arina V Pershina 1 , Darya V Bazovkina 1 , Vladimir S Naumenko 1
Affiliation  

We investigated the effect of chronic (6 weeks) consumption of 10% alcohol on the principal elements of BDNF (BDNF, proBDNF, p75, and TrkB receptors) and 5-HT (5-HT, 5-HIAA, tryptophan hydroxylase-2 [Tph-2], 5-HT transporter [5-HTT], 5-HT1A, 5-HT2A, and 5-HT7 receptors) systems in the brain of C57Bl/6 mice. BDNF mRNA level in the raphe nuclei area and BDNF protein level in the hippocampus were lowered in ethanol-treated mice. The increase in proBDNF protein level in the raphe nuclei area, cortex, and amygdala and the increase of p75 receptor protein levels in the raphe nuclei area were revealed after ethanol exposure. Alcohol intake reduced the protein level and increased the activity of Tph-2, the key enzyme for serotonin biosynthesis in the brain, and increased the main 5-HT metabolite 5-HIAA level and 5-HIAA/5-НТ ratio as well as the 5-HT7 receptor mRNA level in the raphe nuclei area. In the cortex, 5-HT2A receptor protein level was reduced, and 5-HIAA/5-HT ratio was increased.

These data showed considerable impact of alcoholization on the BDNF system, resulting in proBDNF and p75 receptor expression enhancement. Alcohol-induced changes in BDNF and 5-HT systems were revealed in the raphe nuclei area where the majority of the cell bodies of the 5-HT neurons are localized, as well as in the cortex, hippocampus, and amygdala. Our data suggest that the BDNF/5-HT interaction contributes to the mechanism underlying chronic alcohol-induced neurodegenerative disorders.



中文翻译:

关于 BDNF 和血清素系统之间的相互作用:长期摄入乙醇对小鼠的影响。

我们研究了长期(6 周)摄入 10% 酒精对 BDNF(BDNF、proBDNF、p75 和 TrkB 受体)和 5-HT(5-HT、5-HIAA、色氨酸羟化酶-2)的主要成分的影响。 Tph-2]、5-HT 转运蛋白 [5-HTT]、5-HT 1A、5-HT 2A和 5-HT 7C57Bl/6 小鼠大脑中的受体)系统。在乙醇处理的小鼠中,中缝核区的 BDNF mRNA 水平和海马中的 BDNF 蛋白水平降低。乙醇暴露后显示中缝核区、皮质和杏仁核中proBDNF蛋白水平的增加以及中缝核区p75受体蛋白水平的增加。饮酒会降低蛋白质水平并增加大脑中血清素生物合成关键酶 Tph-2 的活性,并增加主要的 5-HT 代谢产物 5-HIAA 水平和 5-HIAA/5-НТ 比率以及中缝核区的5-HT 7受体 mRNA 水平。在皮质中,5-HT 2A受体蛋白水平降低,5-HIAA/5-HT 比值升高。

这些数据表明醇化对 BDNF 系统有相当大的影响,导致 proBDNF 和 p75 受体表达增强。酒精诱导的 BDNF 和 5-HT 系统的变化在 5-HT 神经元的大部分细胞体所在的中缝核区域以及皮层、海马和杏仁核中显示出来。我们的数据表明 BDNF/5-HT 相互作用有助于慢性酒精诱导的神经退行性疾病的机制。

更新日期:2020-06-27
down
wechat
bug