Bladder dysfunction is one of the most common diseases that occur for a number of reasons and the current treatment modalities do not improve much in its recovery process. Tissue engineering in the last two decades has given great hope for the treatment of these disorders. In this study, a composite nanofibrous scaffold was fabricated from chitosan, collagen, and polyvinyl-alcohol polymer blend while curcumin incorporated in scaffold fibers. The scaffold supportive functions from smooth muscle cell differentiation were studied when human-induced pluripotent stem cells were cultured on the scaffolds under differentiation medium. Biocompatibility of the fabricated scaffold increased significantly by incorporating curcumin in the scaffold fibers, where protein adsorption, cell attachment, and viability were increased in the nanofiber/curcumin group compared with the other groups. In addition, the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells upregulated while cultured in the presence of curcumin, but this increase was significantly improved while cells cultured on the nanofibers/curcumin. In addition, αSMA protein in the cells cultured on the nanofibers/curcumin expressed significantly higher than those cells cultured on the nanofibers without curcumin. It can be concluded that smooth muscle cell differentiation of the induced pluripotent stem cells promoted by curcumin and this promotion was synergistically improved while curcumin incorporated in the nanofibers. Graphical abstract.

中文翻译：

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iPS 细胞在掺入姜黄素的壳聚糖/胶原蛋白/聚乙烯醇纳米纤维上的高效平滑肌细胞分化。

膀胱功能障碍是由于多种原因而发生的最常见疾病之一，目前的治疗方式在其恢复过程中并没有太大改善。在过去的二十年中，组织工程为这些疾病的治疗带来了巨大的希望。在这项研究中，复合纳米纤维支架由壳聚糖、胶原蛋白和聚乙烯醇聚合物混合物制成，而姜黄素则掺入支架纤维中。当人诱导的多能干细胞在分化培养基下的支架上培养时，研究了来自平滑肌细胞分化的支架支持功能。通过将姜黄素掺入支架纤维中，制造的支架的生物相容性显着提高，其中蛋白质吸附、细胞附着、与其他组相比，纳米纤维/姜黄素组的活力和活力增加。此外，在姜黄素存在下培养时，干细胞中平滑肌细胞相关基因的表达水平上调，包括 α-平滑肌肌动蛋白 (αSMA)、平滑肌 22 α (SM-22a)、Caldesmon1 和 Calponin1，但是当细胞在纳米纤维/姜黄素上培养时，这种增加得到了显着改善。此外，在纳米纤维/姜黄素上培养的细胞中，αSMA 蛋白的表达明显高于在没有姜黄素的纳米纤维上培养的细胞。可以得出结论，姜黄素促进了诱导多能干细胞的平滑肌细胞分化，并且当姜黄素掺入纳米纤维中时，这种促进作用得到了协同改善。图形概要。